Paper-based microfluidics devices can create a new healthcare model. Cellulose is carbohydrate polymer biocompatible and hydrophilic. These characteristics enhance the development of userfriendly diagnostic devices, but the link between paper manufacturing process and performance of the devices is still unclear. Previous studies focused on either commercial papers or lab papers from wood-cellulose fibers, with different basis-weight. This work introduces the effect of refining process and lab paper from non-wood-cellulose fibers, focusing on sisal fibers to overcome the aforementioned challenge. Structural characteristics of paper, such as basis-weight and degree of refining, are optimized and correlated with blood typing test resolution. Unrefined sisal paper of 50 g/m 2 and 100 g/m 2 basis-weight exhibit a higher gray intensity level than refined paper, and also maximal capillary rise and a pore size suitable for blood grouping tests. Two different blood types were evaluated with results consistent with the traditional methods, testifying the usefulness of this methodology.

Blood typing is especially important in any emergency transfusion and during pregnancy. Transfusion of a non-compatible blood type can carry out severe health problems, since hemoagglutation reaction with opposite antibodies stick cells together.Conventional blood typing test in most countries require direct and reverse blood typing. The second step, called reverse typing is done using the natural antibodies from the patient's plasma. The plasma of blood without cells is mixed with blood that is known to be type A and type B. Persons with type A blood have anti-B antibodies, and those with type B blood have anti-A antibodies. Type O blood contains both types of antibodies. The agglutination of the aforementioned cells will allow the determination of the blood type from the natural antibodies of patient's plasma.Currently, this step is done in clinic labs since it requires a centrifuge to separate plasma from blood. This study presents an approach that combines a microfluidic a blood plasma separator and a paper-based blood type detector.The proposed high throughput blood plasma separator device has been designed to use cross flow filtration in order to extract higher volume of plasma (0.1 μL) from fresh undiluted blood (2 μL) with high purity (100%) in an admissible time (5 min) to implement for blood typing tests.The main advantage of this design is the efficiency and maximization of the amount of obtained plasma from initial sample. The results of this microfluidic system have been compared with commercial lateral flow assays to validate the quality and quantity of the extracted plasma and to prove its capability as a blood back typing input.