Aquaculture procedures usually induce stress that affects the physiological status of fish. For this reason, the inclusion of additives in fish feeds to palliate stress might be a good alternative. This study aimed to assess the antimicrobial activity of citronella (Cymbopogon nardus) essential oil (CEO) against bacterial pathogens and to determine its dietary impact on the growth performance of Sparus aurata. In vitro tests confirmed that CEO possesses antimicrobial activity against several fish-specific pathogens. For the in vivo tests, three experimental groups were fed for 60 days with different concentrations of CEO: CTRL (0 mL kg−1 fish feed); CEO1 (1 mL kg−1 fish feed); and CEO2 (2 mL kg−1 fish feed). At the end of the experiment, the physiological status was characterized. Subsequently, the specimens of the CTRL and CEO2 groups were subjected to a challenge with an injection of Poly I:C for immune stimulation. Although S. aurata individuals tolerated CEO inclusion without compromising growth performance, it significantly reduced glycogen in the CEO2 group, concomitant to an increment of total peripheral leucocytes. Moreover, different hematological profiles’ responsive patterns against an inflammatory stimulus were observed. In conclusion, our results suggest that the use of CEO as a fish feed additive can prevent bacterial outbreaks and improve potential in vivo disease resistance in S. aurata without negatively affecting growth.
The reasons for the inferior performance of many existing buildings and associated energy systems are diverse, but an important part-cause is insufficient attention to the influence of occupant behaviour. In smart buildings it is necessary to allow for the integration of human behaviour in the HVAC system. In addition, many researchers are limited in their investigation by not having low cost tools that can provide information for their studies. This article is a review of the present state of art about the globe thermometer. It describes how to build your own globe temperature sensor and describes experiments that illustrate the feasibility of using a black globe thermometer with 40 mm diameter. ResumenLas razones del rendimiento inferior de muchos de los edificios actuales y sus sistemas energéticos relacionados son diversas y estas son en una parte importante causada por una atención insuficiente a la influencia del comportamiento de los ocupantes. En los edificios inteligentes es necesario implementar nuevas oportunidades para integrar el comportamiento humano en el sistema de climatización. Además, muchos investigadores están limitados en su investigación al no contar con herramientas de bajo coste que puedan proporcionar información a sus estudios. En este artículo se presenta una revisión del estado actual del arte sobre el termómetro de globo, se describe cómo construir su propio sensor de temperatura de globo y los experimentos descritos ilustran la viabilidad de utilizar un termómetro de globo negro con 40 mm de diámetro.Palabras clave: Temperatura de globo, termómetro, temperatura media radiante, hardware abierto, sensor.
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