A study on eggs from both organic and conventional was conducted in order to compare between them. The eggs were evaluated for their physical quality, microbial load, and presence of antibiotic residues. The study revealed that eggs from organic system were smaller (47.8 vs. 64.3 g) with yolk, albumen, and egg shell weights significantly lower than eggs from conventional system (15.2 vs. 19.2g), (25.3 vs. 34.1 g), and (7.12 vs. 9.5 g) respectively. The Yolk/albumen ratio being higher in the organic eggs (0.60 vs. 0.56). The percentage of egg shell was not affected by the housing system (14.8 vs. 14.7%). The overall defects in conventional eggs was higher (40% vs. 16%). The microbiological examination showed that total bacterial and total mold counts were significantly higher in organic eggs, with the shell having the highest and albumen having the lowest microbial load. Monitoring of antibiotic showed zero residues of antibiotics in organic eggs while 8% of conventional eggs had antibiotics residues.

The main objectives of this work: 1-Seasonally collect fish from Mediterranean-sea and studying the clinical picture in the examined fish. 2-Isolation and identification the pathogenic bacteria affecting Solea fish. 3-Registering the percentage of total and seasonal prevalence in such fish. 4-Make trials for diagnosis using recent techniques. 5-Application of biosafety in the lab. 6-Commitment of the basis and principles of scientific research ethics in all study stages. 7-Studying histopathological changes caused by these bacterial diseases from naturally infected fish. In the present study, a total of 120 Egyptian sole (Solea aegyptiaca) with various body weights (50-70 g) and lengths (13-19 cm) were collected seasonally and randomly from costal area of Mediterraneansea in Port Said Governorate, Egypt from September-2019 till August-2020. Moribund or freshly dead specimens collected in aerated airbags and/or iceboxes were transferred to Food Hygiene and Bacteriology Unit, Animal Health Research Institute, Port Said for further bacteriological, and molecular examinations in particular for Vibrio infection. Fish were found to be infected with Vibrio alginolyticus based on the characteristic phenotypic and biochemical profiles with a total prevalence of 29.16%. During the study, the highest incidence of vibriosis among the naturally infected sole fish was observed in the summer season (31.43%), with the prevalence in the spring and winter seasons being equal (25.72% each). In contrast, the lowest incidence of infection was observed during autumn (17.14%). Molecular investigation of pure isolates using specific set 184 Esraa Abdelazeem et al. of primers targeting the 16S rRNA conserved gene of Vibrio species gave expected amplicons size of 663 bp.

was isolated from basturma only in 4 samples (26.6%). On other hand L. monocytogenes could not be isolated from any of the other examined samples. Polymerase chain reaction is a powerful technique for detection of pathogens in foods. It is a rapid procedure with both sensitivity and specificity for quick detection and identification of specific pathogenic bacteria from different sources. Listeria monocytogenes detection methods based on PCR amplification of the prfA and hly genes sequences have been reported. The virulence gene prfA was found in all isolates which is responsible for the initiation of pathogenesis so its target is to detect pathogenic L. monocytogenes. The public health hazard of this microorganism as well as recommended measures to improve quality status of ready-to-eat (RTE) meals was discussed.

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