Raoiella indica Hirst, 1924 (Prostigmata: Tenuipalpidae), is one of the main pest mites of palm and banana trees, causing significant damage in producing regions. Thus, the objective of this work was to evaluate the acaricidal effect of soursop peel extract (Annona muricata L.) on R. indica adults. The experiment was conducted in a completely randomized design using soursop peel extract, with 7 replications and 12 individuals of R. indica per replication. The experimental units consisted of discs of coconut palm leaves (4 cm in diameter), with cotton moistened at the bottom of the Petri dish (10.0 x 1.2 cm) and around the disc to maintain turgor and prevent mites from escaping. The application was carried out using an airbrush, connected to a calibrated compressor with a constant pressure of 1.3 psi and 1 mL of solution per plate. The acaricidal effect was evaluated 24, 48, and 72 hours after spraying. Mortality data were corrected and subsequently submitted to probit analysis (p≤0.05) using the statistical program R, with the LC50 and LC90 of the extract being calculated. The soursop peel extract at the maximum concentration used (15%) showed mortality of 80% of individuals of R. indica. The LC50 was 1.934%. It was concluded that extracts from soursop peel have acaricidal potential on R. indica in the laboratory.
The two-spotted spider mite, Tetranychus urticae Koch, is one of the most important mite species in the world, this specie is related to attacking more than 250 crops of economic importance. This pest can occur year-round but grows best in warmer and drier times. This way, the objective of this work was to evaluate the acaricidal potential of the extract of the jatropha pie (Jatropha curcas L), in different concentrations, aiming for the pest management of T. urticae. The solutions were sprayed under sheets of Canavalia ensiformis, with the support of the Potter tower, consisting of direct application. In the indirect route of application, the leaf discs were immersed in the extract solution of the J. curcas, placed on a paper towel to dry, and subsequently, transferred to Petri dishes, with cotton moistened on the bottom and sides. Females of T. urticae were transferred to leaf discs. Ten replicates were used per treatment, evaluating the mortality as a function of post-spraying time. At all concentrations tested, the direct application treatment was superior to the indirect application, causing greater mortality of the two-spotted spider mite in laboratory conditions.
The aim of this work was to study the effect of the interaction between castor bean oil (Ricinus communis) and jatropha oil (Jatropha curcas) on the aphid, Myzus persicae. The experiment was realized at the Institute Federal of Espírito Santo in Brazil. Tests were conducted in a completely randomized design, with 11 treatments and 10 repetitions, arranged in a factorial arrangement of 11 (interactions between oils) x 2 (application forms). The concentration used in this test was 3 %. Treatment interaction ratios were 0-100; 10-90; 20-80; 30 -70; 40-60; 50-50; 60-40; 70-30; 80-20; 90-10; 100-0 % jatropha and castor bean oil, respectively. Tests of direct and indirect application were performed. The evaluations were performed 24, 48, and 72 hours after the applications. For both forms of application, there were significant mortalities. Mortalities greater than 70 % were observed when the interaction contained more significant amounts of jatropha than castor bean. However, a negative interaction was observed between concentrations with similar proportions. In conclusion, for both application forms, the interaction between jatropha and castor bean oils effectively reduce the population of M. persicae.
The coffee red mite [Oligonychus ilicis (McGregor, 1917)] (Prostigmata: Tetranychidae) is one of the main causes of conilon coffee (Coffea canephora Pierre & Froehn) crop damage due to its phytophagous habit. Nowadays, environment and human harmful synthetic pesticides are used to control this pest. In this context, plant-derived bioactive compounds have been studied as a sustainable alternative for the pest mite management in crops. The aim of this study was to evaluate the castor (Ricinus communis) seed oil action on eggs, larvae, nymph (protonymph and deutonymph) and adults of O. ilicis. Coffee leaf discs (4 cm in diameter) containing 12 individuals of O. ilicis were sprayed with castor seed oil at concentrations of 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0% (v/v) using airbrush. The ovicidal and mortality activity of this extract were evaluated against O. ilicis. The effective concentration of castor oil to cause 50% inhibition of O. ilicis larvae hatching (LC 50) was 1.26% (v/v). Oligonychus ilicis treated with this oil at a concentration of 3.0% presented a larvae hatching percentage of 29.3%, lower than that obtained for the control treatment (79.1%). Castor seed oil 3.0% (v/v) was highly toxic to nymphs and adults of O. ilicis with mortality of 96 and 88%, respectively. Castor seed oil was effective for larvae hatching inhibition and nymphs and adults mortality of O. ilicis, being promising for the coffee red mite sustainable biocontrol.
Tetranychus urticae is an important phytophagous and cosmopolitan pest, affecting several cultures worldwide, such as papaya, strawberry, cotton, beans and soybeans. Chemical pesticides for mite control pollute the environment, poison humans and select resistant mite populations. Ricinus communis cake is a by-product of biodiesel production, containing compounds, such as ricinin and some fatty acids, described as toxic to arthropods. The objective of this work was to evaluate the castor bean cake acaricide potential on the spider mite, T. urticae. Ricinus communis dried seeds were pressed for oil extraction. Subsequently, the cake obtained after the oil extraction was ground in a knife mill to produce a fine powder. After, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 grams of castor bean cake powder were mixed into 100 mL of Tween 80 aqueous solution 0.05 % v v-1, stirred (30 minutes, at 25 °C) and left to rest (20 minutes) to decant the solid particles. Finally, the supernatant (castor cake extract) was separated from the solid part by simple filtration. For direct application, 6 mL of castor cake extract were sprayed onto 10 T. urticae females dispersed on a 4 cm diameter Canavalia ensiformis leaf. For indirect application, 10 T. urticae females were placed in discs leaf, previously immersed in castor bean extract. Ten replicates were used for each treatment, evaluating the mortality parameter as a time function (24, 48 and 72 hours) for both application forms. The obtained data were submitted to linear regression analysis. T. urticae mortality did not show a dose-dependent behavior as a function of the castor bean cake extract concentrations for both forms of application (direct and indirect). Castor bean cake extract at 2.5 % w v-1 caused the highest mortality T. urticae females treated directly or indirectly (95 or 88 %, respectively) than this extract in the other evaluated concentrations. The average mortality of T. urticae females treated directly or indirectly with this extract at the different concentrations evaluated were 88.4 and 77.7 %, respectively. The results showed that the castor bean cake extract, applied directly or indirectly in T. urticae, caused high mortality of females of this mite, being a promising alternative for the spider mite sustainable management.
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