The process of drug development requires a suitable technique which helps the scientist to analyze the drug molecule is an accurate, precise, and easiest way. For the quantitative and qualitative estimation of drugs in analytical chemistry it is very important to identify the best method for method development.  This study helps the author to understand the various analytical techniques available for the process of drug development which includes spectroscopy, chromatography, electrochemical techniques, electrophoretic, flow injection analysis, and hyphenated technique. All these methods contain different analytical process with a variety of separate techniques. Also, we discuss about the modern trend which are available, and implacable in all these methods to improve the analytical behavior of these techniques. In method development process the validation of document must be required in the form of accuracy, precision, specificity, limit of detection, linearity, and range is considered. So, this review article contains the brief summary of available analytical techniques, and the latest trend in method development, or the process of method validation, and development of method. The discussed methods in this review article were revealed by the scientist, and these techniques must require in new drug development process, which helps the person to utilize the potential of these techniques. Trend in the analytical chemistry to overcome the error in method development was necessary, and the latest trends in method development technique were useful to defeat errors in analytical techniques. Keywords: Analytical techniques, Modern trends, Process of method development, and validation.

A simple, sensitive and specific liquid chromatographic method was developed for the simultaneous estimation of Rosuvastatin calcium and Losartan potassium in bulk form. Chromatographic conditions included the  Column C-18 (Shim-pack) 250 x 4.6 mm, particle size 5 µm , mobile phase acetonitrile,  methanol and water pH 3 (orthophosphoric acid) in the ratio 20:25:55 recorded  at 233 nm. The retention time were found to be   3.55 and 4.64 min and average percentage recovery 99.42% and 99.63% for Rosuvastatin calcium and Losartan potassium respectively. The proposed method was found to comply with ICH guidelines. Keyword: Rosuvastatin calcium, Losartan potassium, RP-HPLC, accuracy and linearity.

Chalcones and their derivatives have been an area of great interest for several researchers in recent years. Several number of research publications have been published and chalcones continue to show promising effect for novel drug investigations. Chalcone is an advantaged moiety with therapeutic importance as it comprises of receptive ketoethylenic moiety – CO–CH=CH– having a place with flavonoids. Chalcones (1, 3-Diphenyl-prop-2-en-1-one) consists of a three carbon α, β-unsaturated carbonyl system and two or more aromatic rings and acts as precursors for the biosynthesis of flavonoids in plants. The presence of a highly reactive α, β-unsaturated carbonyl system in chalcone and its derivatives is the justification for its pharmacological potencies. However, synthesis in laboratory of broad range of chalcones has also been reported. Chalcones show a wide range of pharmacological impacts like anthelmintic, antileishmanial, antifungal, antimalarial, antioxidant, antiviral, antibacterial, antiulcer, antimycobacterial, insecticidal, antigout, antihistaminic, antiprotozoal, insecticidal, anticancer, antidiabetic, anti-inflammatory, analgesic etc. Chalcones can be synthesized through Claisen–Schmidt's condensation, Heck's reaction, Aldol condensation reaction, Suzuki's reaction, from cinnamic acid, Sonogashira Isomerization Coupling reaction, Microwave assisted synthesis etc. The purpose of the present review is to centralize the various and widely employed methods of synthesis of chalcone and their various derivatives and their antimicrobial and antioxidant activities.

A rapid isocratic chromatographic procedure for the analysis of methotrexate, hydroxychloroquine sulfate in bulk drug and pharmaceutical formulations was developed validated in the present study. The mobile phase consists of a mixture of Water: Acetonitrile: Tetrahydrofuran in the proportion of 50:40:10 and pH maintained to 3with perchloric acid. Retention time was found to be 3.0 and 3.7minutes for methotrexate and hydroxychloroquine sulfate respectively. The method was performing by using the C18 column, ODS Hypersil column with UV detection at 318nm, and flow rate of 1.0ml/min. The percentage of recovery for both drugs was found to be 99.99%. All validation parameters were within limits as per the ICH guidelines.

A simple, sensitive, economic and specific reverse phase liquid chromatographic method was developed for the simultaneous estimation of Emtricitabine and Ritonavir in bulk drug. Chromatographic conditions consisted of   C-18 Column (Shim-pack) 250 x 4.6 mm, particle size 5 µm , mobile phase combination of methanol and water (80:20), flow rate 1ml per minutes, run time 15 minutes and UV detection at 251nm. . The retention time for Emtricitabine and Ritonavir were found to be   3.25 and 7.8 min and average percentage recoveries 99.42% and 99.63% respectively. The validation parameters were found to comply with ICH guidelines. These methods can be further employed in future for the routine determination of Emtricitabine and Ritonavir in bulk drug and formulation. Keyword: Emtricitabine, Ritonavir, RP-HPLC, accuracy and linearity.