Samples of soil, forage and blood serum of cattle in six villages Ganderbal district of kashmir were collected and analysed for different macro and micro mineral contents to establish the mineral correlation among soil, forage and animals. The macro and micro mineral contents in soils of Ganderbal district were higher than their respective critical levels except Mn. Similarly, in forage except for zinc all the mineral values were above critical level. The study also revealed the serum mineral values above the critical levels. The soil and forage (r=0.558) and forage and serum (r=0.463) showed significant positive correlations while, non-significant (P<0.05) positive correlation (r=0.08) between soil and serum for Cu was observed. The correlation for Calcium, phosphorus, magnesium, zinc, iron and manganese observed between soil-forage, Forage-serum as well as soil-serum was non-sgnificant. Based on present study, supplementation of calcium, phosphorous, copper and zinc in the diet of cattle under existing feeding practices in Ganderbal district of Kashmir is imperative for better health and productivity. However, further, studies should elucidate the bioavailability and strategic dietary supplementation of minerals for livestock

The current study was devised to develop alternative, nonantibiotic, and economically viable treatments for bovine mastitis. The concentration of 6.25 μg/mL (25 nm) CuNPs was selected as intramammary (IM) treatment in S. aureus-induced mastitis in rats as this concentration showed a significant zone of inhibition through the in vitro sensitivity test and minimal cell toxicity on fibroblast cell lines. After, this in vivo study was conducted, and rats were divided into four groups of 6 rats each: group I (healthy control/deionized water), group II (disease control), group III (CuNPs), and group IV (gentamicin). Injection of gentamicin IM for 5 days was selected on the basis of an antibiotic sensitivity test. The therapeutic efficacy of CuNPs was assessed on the basis of clinical signs, mammary gland architecture, bacterial load, oxidative stress parameters, and histopathology of mammary glands. The clinical signs of mastitis in group III ameliorated within 3 days of treatment while in group IV clinical signs ameliorated within 4 days of initiation of treatment. On the 5th day after randomization, bacterial load, mammary gland weights, TOS (Total Oxidant Status), and OSI (oxidative stress index) were significantly lower in the CuNPs group compared to the disease control group and commercial antibiotic group. Similarly, TAS of group III was significantly higher compared to that of groups II and IV indicating that CuNPs have better ameliorative efficacy in mastitis. Treatment with IM, CuNPs @ 6.25 μg/mL showed early recovery, reduced bacterial loads, and amelioration of oxidative stress indices henceforth resulting in marked amelioration in histopathological changes compared to rats in group IV. From this study, it may be concluded that CuNPs may provide a potential alternative therapeutic regimen for the treatment of bovine mastitis.

Background: Mastitis is one of the most economically important diseases of dairy animals causing heavy losses in terms of fall in milk production, decreased milk quality and additional costs for veterinary services. Mastitis is caused by a wide variety of microorganisms including bacteria, fungi, yeast and mycoplasma, of which bacteria are the most frequently isolated pathogens. Microbiological testing is necessary for identification of cause of mastitis and adaptation of control measures to prevent the disease. The aim of the presnt study was to isolate and identify major mastitis causing bacteria from clinical mastitic cases presented to Veterinary Clinical complex, FVSc. and AH, Shuhama Kashmir for treatment. Methods: A total of 200 bovine milk samples from CMT confirmed clinical mastitis cases were collected and cultured on Nutrient agar, Blood agar, Mac-Conkey agar, Eosine Methylene Blue (EMB) agar, Mannitol salt agar and Edwards media for bacterial isolation. The isolates were then subjected to biochemical tests for identification. Result: Upon microbiological testing and biochemical confirmation, the major mastitis causing bacteria isolated from clinical cases of Bovine mastitis were Staphylococcus Spp. showing highest prevalence of 46.4% followed by mixed infection with Staphylococcus and Streptococcus (20.8%), Streptococci Spp. (18.4%) and Escherichia coli (14.4%).

The study was conducted to find out the activity of some medicinal plants against bacterial isolates. The bacterial isolates from milk samples of infected quarters of cows were investigated for in-vitro drug sensitivity by standard disc diffusion technique (Bauer et al., 1966). Cultural examination of milk samples was done by method described by Quin et al. (2004) in which predominant isolates were Staph. (55.55%), E. coli (22.22%), Strepto. agalactiae (16.66%) and other isolates as 5.5% and were subjected to in-vitro antibacterial sensitivity test to selected herbal extracts and standard antibiotic (cefuroxime) (Table-1). The four different aqueous concentrations of the herbs namely Fumaria indica, Adiantum capillus, Nepata cataria, Levandula stoeches and Borago officinalis collected from registered herbal shops prepared by standard procedure as 25mg/ml, 50mg/ml, 75mg/ml and 100mg/ml were used. The results indicated that aqueous extract of Fumaria indica, Adiantum capillus and Napeta cataria against Staphylococcus aureus, E.coli, Streptococcus agalactia and K. pneumonia exhibited maximum zone of inhibition 20.0±1.21, 21.0±0.19, 13.0±0.37 17.0±0.21; 18±0.41, 12±0.21, 13±0.31, 15±0.31 and 15±0.33, 16.01±0.19 14.09±0.37, 13.31±0.41 at 100 mg/ml respectively which was significantly low as compared to standard drug (cefuroxime) at 30 microgram concentration. Aqueous extract of Levandula stoeches against Staphylococcus aureus and E. coli exhibited maximum zone of inhibition 18.0±0.33 and 13.0±0.141 at 100 mg/ml respectively. Borago officinalis has shown nil to non-significant bacterial growth inhibition activity.

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