No abstract

It was hypothesized that dimethyleacetamide (DMA) can be used as an alternate to glycerol for cryopreservation of Indian red jungle fowl semen. Four concentrations of DMA (4%, 6%, 8% and 10%) in extender were compared with previously optimized cryopreservation protocol based on 20% glycerol (control) for Indian red jungle fowl. Sperm motility, plasma membrane integrity, viability, and acrosome integrity were assessed at the stage of post-dilution, cooling, equilibration, and freeze-thawing. The whole experiment was repeated/replicated for five times independently. Sperm motility, plasma membrane integrity, viability and acrosome integrity were recorded highest (P < 0.05) at post-dilution, cooling, equilibration, and freeze-thawing in extender having 6% DMA compared to control and other experimental extenders. The highest (P < 0.05) recovery rates of all aforementioned parameters were also recorded in extender having 6% DMA; thus, 6% DMA was further compared with control (20% glycerol) for fertility after artificial insemination. Eggs were collected for five days after artificial insemination with semen cryopreserved in extender containing 6% DMA and control. The higher no. of fertilized eggs, fertility, no. of hatched eggs, hatch (%) and hatchability were recorded with semen cryopreserved in extender having 6% DMA compared to control. It is concluded that 6% DMA maintained higher post-thaw quality and fertility of Indian red jungle fowl semen and is a better replacement of glycerol.

Agriculture is one of the greatest threats to biodiversity worldwide, but knowledge of how agriculture modifies animal movement, which is crucial for survival, is limited. Here, we examined the effect of landscape composition on the movements of the oriental garden lizard Calotes versicolor in agricultural landscapes of north‐central Pakistan. We radio‐tracked 32 individuals over 5 months to determine whether land cover type (farmland, tree patches, grassland) influences hourly movement rate, inter‐day distance moved, and activity area size. We found that hourly movement rates were higher in tree patches compared to grasslands, and higher when animals moved between land cover types rather than within individual land cover types. Activity area size and movement rates became smaller as the season progressed, but they did not differ according to animal sex or body size. Habitat selection analysis showed that lizards preferred tree patches, avoided farms, roads, water bodies and human dwellings, and used grasslands in proportion to availability. When lizards used farmlands, they were found in field margins 85% of the time. Our results emphasize the importance of treed areas as reptile habitat in these highly modified agricultural lands. Agricultural intensification that reduces the availability of tree patches and field margins will likely reduce the extent to which lizards can use the landscape by removing preferred habitat. Maintaining tree cover and small fields with field margins should promote the coexistence of wildlife conservation and food production in agricultural landscapes.

Black Drongo is an insectivorous bird native to Pakistan. The present study focused on its feeding preferences, behaviour, ecology and habitat relationships. The study was carried out from first week of December, 2019 to the second week of April, 2020 in the two villages of Rawalpindi named as Daultala (Location-I) and Nata Gujarmall (Location-II). Observations were made by point count method on foot using line transect method. Air (52.27%) was the most commonly used feeding substrate. Aerial feeding mode (52.27%) is preferred over plant or tree gleaning (n=58) and ground feeding (n=47). Most common perching site was man made obstacle, wire (37.72%). Thirteen indigenous tree species and two exotic trees including Eucalyptus (Eucalyptus camaldulensis) and Bottle brush (Callistemon citrinus) were used as perching site by Black Drongo throughout the study. Black Drongo remained solitary (36.36%) during feeding rituals and shared (63.63%) its intimate habitat with other birds. At Location-I and Location-II, habitat was shared with 12 and 16 bird species, respectively. The mean time of prey capturing attempt was lower at Location-I (1.93±0.17 min) than Location-II (2.16±0.16 min). The time range of prey capturing attempt was (0.1±0.16 min) to (11.5±0.17 min). Black Drongo captured prey successfully by first attempt (59.54%), maximum attempts noted were 6. After preying attempts Black Drongo came back to same perch site (67.27%); which is a representative of resource abundance.

Rose-ringed parakeet (P. krameri) is commonly found native psittacid in Pakistan. It is most popular companion bird in Pakistan. It is an unprotected species as per The Punjab Wildlife Act, 1974. The objectives of the present study were to study its population paradigm and basic nesting biology in the Gujar Khan, Punjab, Pakistan. Previously, no studies were carried out to assess its live and vacant nest cavities, nesting site, nest height, cavity position on substrate, and potential roosts in the area. Moreover, this study also assesses the potential threats and factors in this area and conservation of the parakeet. Results show that in the study area (36.77%) active and (63.22%) inactive nests were found. A total of 10 tree species were utilized to make nests. It was found Ficus bengalensis, Syzygium cumini, Morus alba, Melia azedarach and Broussonetia papyrifera as the most used tree species for nesting due to more frequencies and old ages. Parakeets make nest at 3-11 m height. The preferred nest height (42.48%) was in the range of 7-9 m, while (5.88%) least at 3-5 m height. For safety reasons they made more nests (45.09%) on the tree trunk followed by terminal (31.37%) and at fork (23.52%). Due to smaller thriving population we found only (mean=7±4) parakeets during roost counting. Severe cutting of trees, destruction of its habitat and poaching for selling in the bird market are the main causes of its population decline. Government must change its status from unprotected to protected species and should ban its dealing for pet.