This is a pilot study, which is intended to represent a basis for future studies pertinent to multidrug-resistant antimicrobial gram-negative (MDR-GNB) bacteria that commonly prevail in Iraqi community and hospital settings.In the present study, we scrutinize the antimicrobial phenotypic resistant patterns of commonly isolated Enterobacteriaceae (i.e., E. coli, E. aerogenes, K. oxytoca, P. mirabilis) from urine, wound samples, and high vaginal swabs in the Iraqi community. One hundred fifty-six (156) urine, genital lesions, and high vaginal swab samples were obtained from a community population in a cross-sectional study held in Baghdad at a private Lab from December 2020 to the end of April 2021. These specimens were isolated and identified according to conventional methods. Antimicrobial sensitivity tests were done according to the Kirby-Bauer method, and interpretation of results was made with reference to CLSI Standards for Antimicrobial Susceptibility Testing. Gram-negative bacilli were isolated from 80 (about 51%) of genitourinary specimens sent to the laboratory for culture and sensitivity. E. coli and E. aerogenes showed the most frequent bacterial isolate in genitourinary samples (60% and 31.25%, respectively). Complete resistance (i.e. 100%) to Amoxicillin-Clavulanic acid and Cefotaxime and high resistance (i.e. 97.5%) to Cefixime, Ceftriaxone, and Ceftazidime by these bacterial strains were observed. Assessment of Enterobacteriaceae antibiotic resistance for both of ß-lactams and Carbapenems showed that 25% of these strains were having resistance to both groups of antibiotics. As a conclusion; Enterobacteriaceae multi-drug resistance reflects potential ESBL, Ampc, and carbapenemase activity in Iraq.