No abstract

ABSTRACT. Distribution of estrogen receptor alpha (ERα) in the dominant follicle (DF) and corpus luteum (CL) at the three stages of estrous cycle in Japanese Black cows was evaluated by means of immunohistochemistry. Ovarian dynamics were observed twice daily using ultrasonography until the ovariectomy performed on Day 7 (First group, n=3), Day 10 (Second group, n=3) and Day 18 (Third group, n=3) (Day 0=estrus). Expression of ERα represented by immunohistological staining intensity in cells was determined using a light microscope equipped with a digital camera. A tendency toward higher expression were observed in theca interna (TI) of DF when compared with those in mural granulosa cells (mGC), antral granulosa cells (aGC) and theca externa (TE). ER α expression in the Third group was lower than that in the First Group in mGC, and it was also lower than that in the second group in TE (P<0.05). ER α expression in luteal cells was higher than those in the stromal cells in CL. No significant difference of ERα expression was observed within luteal or stromal cells, except in the Second group in the luteal cells, in which significantly higher expressions than that in the Third group (P<0.05) were observed. The results showed that, 1) ERα was present in developing DF on Day 7, early regressing DF on Day 10 and preovulatory DF on Day 18, especially in the TI, and a few were localized in the mGC, and 2) ER α was highly expressed in the luteal cells and the expression decreased in combination with regression of CL. KEY WORDS: corpus luteum, dominant follicle, estrogen receptor alpha, immunohistochemistry, Japanese Black cow.

Penelitian ini bertujuan untuk mengetahui perkembangan folikel sapi PO dara yang distimulasi menggunakan PMSG dosis rendah (dosis non-superovulasi) dan perolehan folikel dominan pre-ovulatori sebagai informasi dasar untuk penerapan intrauterine programming pada sapi. Sapi PO dara berjumlah sembilan ekor dibagi ke dalam tiga kelompok dosis PMSG yaitu kontrol (NaCl 0.9% sebagai placebo), dosis PMSG 0.5, dan 1.0 IU/kg BB. Injeksi PMSG dilakukan pada awal gelombang folikel ke-2 berdasarkan perubahan dinamika ovari yang dikonfirmasi menggunakan USG diikuti injeksi PGF2α 48 jam kemudian. Perkembangan folikel sejak injeksi PMSG hingga terbentuknya pre-ovulatori folikel (POF) diamati dan dipetakan menggunakan USG. Jumlah POF tertinggi terdapat pada dosis PMSG 1.0 IU/kg BB (4.67+1.67) secara signifikan (P<0.05), sedangkan POF yang terbentuk pada dosis PMSG 0.5 IU/kg BB berjumlah satu, sama dengan kelompok kontrol (P>0.05). Meskipun jumlah POF yang terbentuk berjumlah satu, namun rataan diameter dan volume kelompok sapi dosis 0.5 IU/kg BB lebih besar 16.13% dan 57.14% secara berurutan dibandingkan kelompok kontrol, diikuti oleh dosis 1.0 IU/kg BB dengan nilai tertinggi secara signifikan (P<0.05). Berdasarkan hasil tersebut dapat diketahui bahwa PMSG 0.5 IU/kg BB dapat terkontrol sehingga dapat digunakan sebagai metode pendekatan intrautering programming pada hewan monotokus.

Gnathostoma doloresi, a nematode parasite producing ulcerative and/or granulomatous lesions in the gastric wall of domestic pigs and wild boars (Sus scrofa leucomystax) throughout southeastern Asia, is an important zoonosis causing cutaneous larva migrans in humans. Patients infected with G. doloresi have been found yearly in the vicinity of Miyazaki Prefecture, Japan. Adult nematodes were found in 31 of 32 wild boars captured at two different geographic locations in the prefecture, a location in the high mountains and one at sea level. The parasite-free animal had gastric lesions typical of a previous infection with this parasite. The nematode intensities were higher in wild boars caught in the mountains than those caught at near sea level. The extremely high prevalence of G. doloresi in wild boars indicates that the life cycle of this parasite is well established in this area of Japan.

<p class="abstrak2">Superovulation treatment on PO cattle (Bos indicus) was less responsive compared to Bos taurus breed. It might due to the difference of their follicular dynamic. This study was conducted to investigate the follicular dynamics and its repeatability in PO cattle. Follicular dynamics observations conducted on 9 cows trough ultrasound scanning every day. Observations of wave patterns repeatability were performed in 6 cows which its wave pattern already known on the next consecutive IOI.  Research result indicated that PO cattle had 3 (66%) and 4-waves (34%) pattern. The first wave of 3 and 4-waves pattern emerged on day -0.4+0.9 and 1.4+1.1 respectively.  The second wave of 3 and 4-wave pattern emerged on day 9.8+1.5 and 7.4+1.9 respectively.  The pattern of 3 waves has a longer follicle dominant duration (11.6+1.5 day) in the first wave of estrous cycle, compared with 4 waves pattern (10+2.92 and 7+1.00 day respectively). The growth rate of dominant follicle was not different significantly between the 3 and 4-waves pattern (0.87+0.23 and 0.94+0.25 mm/day respectively). Similarly, ovulatory follicle diameter between 3 and 4-waves pattern was also not different significantly (12.24+12.34 and 12.30+12.23 mm respectively). Observation of wave patterns repeatability in 6 PO cows indicated that PO cattle had high repeatability in follicular wave pattern (0.88) and the number of growing follicle was 0.91.  This study resulted data for dynamic of follicular development, wave pattern, its repeatability which be expected to design the protocol of superovulation treatment or other reproduction technologies based on follicular dynamic to improve its result in PO cattle.</p><p> </p>