Background
Herbal medicinal material and product need is increasing, and with this increase in the need, it is very much an essential requirement to maintain the quality of them.
Main body
The quality of the herbals is altered by various physical, chemical, and geographical aspects which contribute to the quality of these materials. Apart from that, adulteration is also an increasing concern when it comes to herbal material quality. Various chemical and phytochemical test, analytical techniques, and hyphenated analytical techniques are used for determining the quality aspects of the herbal materials in the herbal pharmaceuticals.
Conclusion
These techniques can be used as quality control tool in assessing the quality of herbal materials and herbal pharmaceuticals.
Background
Traditional medicine being ethnic is preferred worldwide even in these modern days. Obesity is a lifestyle disorder. Many chemically synthesized medicines are available. Poly-herbal medicines can be one of the safest alternatives with less side effects in treating obese patients.
Results
The in vitro anti-lipase activity was carried out for a different concentration. The formulation of the poly-herbal tablets was designed using the Design Expert software. The pre-compression and post-compression studies show that the formulation F6 showed better results of all the formulations designed. Stability study results showed that the poly-herbal tablets were stable throughout the studies.
Conclusion
The results show that F6 is the better formulation based on the tablet evaluation, and all the extracts showed inhibitory activity against pancreatic lipase indicating its active role in the treatment of obesity.
A new method has been developed and validated for the simultaneous estimation of gallic acid and eugenol for the poly-herbal formulation. The HP-TLC (High Performance Thin Layer Chromatography) method was developed using pre-coated silica gel 60 F254 on aluminum plate and the solvent system used was iso propyl alcohol : hexane: ethyl acetate: glacial acetic acid (50:30:30:1 V/V) and scanned at 254 nm wavelength. The Rf value for gallic acid was 0.608±0.041 and 0.752±0.035 for eugenol. The range of linearity detected for gallic acid and eugenol was 2-10 ng µL-1. The method was validated in accordance with ICH (International Council for Harmonization) guidelines for linearity, range, LOD, LOQ, precision, specificity and robustness. The developed method was found to be easy, simple, selective, specific and robust.
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