Analytical method development and validation for estimation of chrysin in chrysin loaded phytosomes using high performance thin layer chromatography

Kudatarkar, Namit; Jalalpure, Sunil; Balekundri, Amruta; Kurangi, Bhaskar

doi:10.1080/10826076.2022.2038196

Cited by 11 publications

(4 citation statements)

References 18 publications

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“…Furthermore, the method parameters, including linearity, precision, accuracy, LOD, LOQ, and recovery, were all determined. [24][25][26][27][28]…”

Section: Hptlc Methods Validationmentioning

confidence: 99%

Untitled

2023

IJGP

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Background: Leptadenia reticulata (Wight & Arn.) has made important contributions to both traditional and modern medicine. Aims and Objectives: The study aims to develop and validate an high-performance thinlayer chromatography (HPTLC) method for the determination of quercetin content in L. reticulata collected from various parts of the country and to assess its anti-inflammatory activity using the bovine serum albumin (BSA) inhibition method. Materials and Methods: Five samples were obtained from different geographical locations in India, and no significant variation was found in the morpho-anatomical study. HPTLC method developed using a toluene: chloroform: ethyl acetate: formic acid (2.5: 2: 4.5: 1) mobile phase at a wavelength of 300 nm. Second, anti-inflammatory activity of L. reticulata extract was assessed using the BSA inhibition approach. Results: For the standard and sample, R f value of quercetin (0.49) was observed. Concentration range of quercetin (60-160 ng/spot) was determined to be linear for the linearity approach, with a regression coefficient for quercetin of 0.999 and an optimal recovery of 101.70%. The limits of detection and limits of quantification were found to be 0.78 and 2.36 ng, respectively. Study revealed quercetin content differed among L. reticulata collected from different geographical regions. The highest and lowest quercetin contents in L. reticulata were detected in LR-103 and LR-099, respectively. From the results obtained, sample LR-103 showed effective anti-inflammatory activity compared to LR-099 at certain concentrations. Conclusion: The developed method is suitable for the detection and quantification of the lowest content of quercetin in L. reticulata. The obtained data indicate that geographic origin and climatic conditions may be major factors in determining the phytocompounds and biological activities of L. reticulata plant species.

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“…Furthermore, the method parameters, including linearity, precision, accuracy, LOD, LOQ, and recovery, were all determined. [24][25][26][27][28]…”

Section: Hptlc Methods Validationmentioning

confidence: 99%

Untitled

2023

IJGP

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“…Nanoformulation for hesperidin estimation [9][10][11][12][13] Thin film hydration was used to create a hesperidinentrapped topical nanoformulation. Briefly, Weigh the exact amount of drug (30mg) and dissolve in 5ml of methanol before sonication.…”

Section: Preparation Of Plant Extracts and Ayurveda Formulation For E...mentioning

confidence: 99%

Development and Validation of Stability Indicating UV-Spectrophotometric Method for the Estimation of Hesperidin in Bulk Drugs, Plant Extract, Ayurveda Formulation and Nanoformulation

Chimagave¹,

Jalalpure²,

Patil³

et al. 2022

IJPER

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Introduction:In present work attempt has been made to develop and validate a simple and sensitive UV spectrophotometric method for estimation of Hesperidin from bulk drugs, plant extract, Ayurveda formulation and novel nanoformulation. Hesperidin is a heteropolycyclic aromatic bioflavonoid found abundance in citrus plants that has been developed into a topical nanoformulation for the treatment of anti-inflammatory activity. It has numerous anti-inflammatory, anti-cancer, and anti-arthritis effects. Objectives: To develop simple, precise, robust, sensitive and accurate UV-Spectrophotometric method for the estimation of hesperidin in bulk drugs, Plant extract, Ayurveda formulation and nanoformulation. Materials and Methods: The optimum condition for the analysis of the drug was established with methanol and water as solvent (1:1). Maximum absorption wavelength was found to be 284 nm with line equation y = 0.0672x +0.003. It showed linear response between the concentration ranges of 2-10 µg/ml. The linear regression coefficient was found to be 0.999. The method was validated for linearity, precision, ruggedness, specificity, sensitivity as per ICH guidelines and all the values of validation was found to be within the acceptance. Conclusion: The developed method was proved to be new, simple, consistent, and accurate it can be used in the pharmaceutical industry as a routine quality control parameter for raw material and formulation.

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“…A 0.25 m membrane filter was used to filter the solution. After filtering, samples of orange and lime peel extract (filtrate) were used in the HPLC analysis Kudatarkar et al,2022;Kurangi and Jalalpure, 2020;Kurangi et al, 2021;Sharma et al,2022).…”

Section: Commercial Orange Peel and Lime Extracts Samplesmentioning

confidence: 99%

Development and validation of stability indicating RP-HPLC method for estimation of hesperidin in nanotransferosome and Madhiphala rasayana—An Ayurvedic marketed product

Chimagave¹,

Jalalpure²,

Patil³

et al. 2022

J App Pharm Sc

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Hesperidin (4′-methoxy3′,5,7-trihydroxy flavanone) is a bioflavonoid molecule with remarkable wound healing properties as well as anticancer, antibacterial, antidiabetic, hepatoprotective, anti-inflammatory, and chemoprevention action. As a result, the current study used a stability-indicated reverse phase-high performance liquid chromatography (RP-HPLC) approach to synthesize and analyze hesperidin nanotransferosome. The important elements of the RP-HPLC method for hesperidin-based nanotransferosome stability highlighted were the retention time of 5.45 minutes, the limit of detection (LOD) of 0.008 μg/ml, the limit of quantification (LOQ) of 0.024 μg/ml, and the % relative standard deviation (RSD) of less than 2%. Hesperidin-loaded nanotransferosome were successfully demonstrated using the current RP-HPLC method, which has been proven to be stable. All vital factors, such as accuracy, linearity, LOD, LOQ, precision, and % RSD, were within the appropriate limits. Hesperidin was successfully quantified and estimated using the specified RP-HPLC method, and hesperidin-based nano transferosome confirmed hesperidin stability. This approach will be vital in evaluating the presence of hesperidin-containing therapeutic formulations.

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Analytical method development and validation for estimation of chrysin in chrysin loaded phytosomes using high performance thin layer chromatography

Cited by 11 publications

References 18 publications

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Development and Validation of Stability Indicating UV-Spectrophotometric Method for the Estimation of Hesperidin in Bulk Drugs, Plant Extract, Ayurveda Formulation and Nanoformulation

Development and validation of stability indicating RP-HPLC method for estimation of hesperidin in nanotransferosome and Madhiphala rasayana—An Ayurvedic marketed product

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