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PurposeTo analyze how ocular surface parameters correlate to presence of pterygium and investigate the possible impact of pterygia on tear film findings and meibomian glands findings.MethodsWe investigated objective parameters of the ocular surface such as conjunctival hyperemia, tear film stability and volume, meibomian gland dysfunction, dry eye disease, corneal topography comparing healthy individuals and correlating with the pterygium clinical presentation.ResultsA total of 83 patients were included. Corneal astigmatism induction was 2.65 ± 2.52 D (0.4–11.8). The impact of pterygium on the ocular surface parameters compared to matched controls was seen in: conjunctival hyperemia (control 1.55±0.39/pterygium 2.14±0.69; p = 0.0001), tear meniscus height (control 0.24±0.05 mm/pterygium 0.36±0.14mm; p 0.0002), meiboscore lower eyelid (control 0.29±0.64/pterygium 1.38±0.95; p 0.0001) and meiboscore upper eyelid (control 0.53±0.62/pterygium 0.98±0.75; p = 0.0083). We found a high number of pterygium patients (88%) presented meibomian gland alterations. Interestingly, meibomian gland loss was coincident to the localization of the pterygium in 54% of the upper and 77% lower lids.ConclusionPterygium greatly impacts on ocular surface by inducing direct alterations in the pattern of meibomian glands besides corneal irregularities, conjunctival hyperemia and lacrimal film alterations, inducing significant symptoms and potential signs of dysfunction.

Background Diseases of the anterior segment of the eye may present different mechanisms, intensity of symptoms, and impact on the patients’ quality of life and vision. The tear film is in direct contact with the ocular surface and cornea and can be easily accessed for sample collection, figuring as a promising source of potential biomarkers for diagnosis and treatment control. This study aimed to evaluate tear proteomic profile in 3 distinct ocular diseases: keratoconus (corneal ectasia), severe dry eye related to graft-versus-host-disease (tear film dysfunction and ocular inflammatory condition) and pterygium (conjunctival fibrovascular degenerative disease). Methods Tear samples were collected from patients of each condition and a control group. By using mass spectrometric analysis combined with statistics and bioinformatics tools, a detailed comparison of protein profile was performed. Results After Student’s t-test analyses comparing each condition to the control group, we found the following number of differentially expressed proteins: 7 in keratoconus group, 29 in pterygium group, and 79 in GVHD group. Following multivariate analyses, we also report potential candidates as biomarkers for each disease. Conclusions We demonstrated herein that mass spectrometry-based proteomics was able to indicate proteins that differentiate three distinct ocular conditions, which is a promising tool for the diagnosis of ocular diseases.

The purpose of this article is to analyze the outcomes of two surgical techniques to treat major trichiasis. A retrospective chart review of 67 patients (89 eyelids) with major trichiasis was performed who underwent surgical treatment using one of two techniques: intermarginal split lamella with graft (ISLG group) or lid lamella resection (LLR group). There were 30 lids in the ISLG group with mean patient age of 71.8 years and 63.3% were females. There were 59 lids in the LLR group with mean patient age of 72.5 years and 52.5% were female. The minimum postoperative follow up was six months. Statistical analysis included descriptive measures, Goodman association test for contrasts between and within multinomial populations and nonparametric Mann-Whitney test for comparison between groups. P < 0.05 was considered statistically significant. The underlying causes of trichiasis were blepharitis (37.07%), chronic meibomitis (21.3%), multiple causes (20.2%), ectropion (11.2%), actinic keratosis (6.7%), or prior ocular surgery (3.3%). Postoperatively, in the ISLG group, there were 20% lids with complete success, 50% underwent laser or electrolysis, 16.7% required further surgery, and 13.3% were unsuccessful. Postoperatively, in the LLR group, there were 47.5% eyelids with complete success, 46.7% underwent laser or electrolysis, 6.8% required further surgery, and 5.1% were unsuccessful. There was a higher statistical chance of complete success with LLR (P < 0.05). LLR is superior to ISLG surgery for the treatment of major trichiasis. There is a greater chance of success with LLR and it is technically simpler.

2627 Purpose: To analyze how ocular surface parameters correlate to pterygium and investigate the 28 possible impact on tear film and meibomian glands. Methods: we investigated objective parameters 29 of the ocular surface such as conjunctival hyperemia, tear film stability and volume, meibomian gland 30 dysfunction, dry eye disease, corneal topography comparing healthy individuals and correlating with 31 the pterygium clinical presentation. Results: A total of 83 patients were included. Corneal 32 astigmatism induction was 2. 65 ± 2.52 D (0.4-11.8). The impact of pterygium on the ocular surface 33 parameters compared to matched controls was seen in: conjunctival hyperemia (control 34 1.55±0.39/pterygium 2.14±0.69; p=0.0001), tear meniscus height (control 0.24±0.05 mm/pterygium 35 0.36±0.14mm; p 0.0002), meiboscore lower eyelid (control 0.29±0.64/pterygium 1.38±0.95; p 36 0.0001) and meiboscore upper eyelid (control 0.53±0.62/pterygium 0.98±0.75; p=0.0083). We found 37 a high number of pterygium patients (88%) presented meibomian gland alterations. Interestingly, 38 meibomian gland loss was coincident to the localization of the pterygium in 54% of the upper and 39 77% lower lids. Conclusion: Pterygium greatly impacts on ocular surface by inducing direct 40 alterations in the pattern of meibomian glands besides corneal irregularities, conjunctival hyperemia 41 and lacrimal film alterations, inducing significant symptoms and potential signs of dysfunction. 3 42 Introduction 43 Pterygium is a degenerative fibrovascular disease of the ocular surface that can cause 44 symptoms of discomfort, corneal irregularities, aesthetics issues thus compromising visual acuity and 45 patients` quality of life. [1-3] The prevalence of pterygium varies worldwide. Global prevalence was 46 estimated in 10.2% to 12%, reaching higher numbers in tropical regions. Several risk factors have 47 been associated with pterygia, such as geographical latitude, residence in rural areas, old age, race, 48 sex, sun exposure, chronic irritation and inflammation. [4,5] 49 Some studies have pointed tear film and ocular surface changes related to pterygium, but 50 consistent correlations remain unknown. [6-9] Although numerous theories have been implicated in 51 pterygium pathogenesis, including ultraviolet radiation exposure, viral infection, oxidative stress, 52 genetic issues, inflammatory mediators, extracellular matrix modulators, it remains controversial as 53 well as its impact on ocular surface homeostasis and function.[10] And a better understanding of the 54 pathophysiological mechanisms associated with pterygium, the morphological alterations on the 55 ocular surface and functional impact may contribute to specific approaches and more effective 56 therapeutic proposals for this common ocular condition. 57 This study aimed to evaluate how ocular surface parameters correlate with pterygium clinical 58 presentation and its impact on ocular surface structures and homeostasis. 4 59 Materials and Methods 60 The present study had a transversal, obs...