The adequate interpretation of facial expressions of emotion is crucial for social functioning and human interaction. New methods are being applied, and a review of the methods that are used to evaluate facial emotion recognition is timely for the field. An extensive review was conducted using the Web of Science, PsycINFO, and PubMed databases. The following keywords were used to identify articles that were published within the past 20 years: emotion recognition, face, expression, and assessment. The initial search yielded 291 articles. After applying the exclusion criteria, 115 articles were included in this review. Articles were analyzed using 3 different approaches: (a) non-behavior-dependent methodologies (MRI and electroencephalography [EEG]), (b) behavioral instruments that were used to assess facial emotion recognition, and (c) instruments or methods that were used to assess facial recognition impairment in health conditions. The behavioral instruments were further subdivided into 7 groups. Static human face stimuli were the most frequently used method, although there are some criticisms concerning the ecological validity of these types of stimuli. Nonvalidated instruments were also commonly used, especially in psychopathological studies. Computer-based morphing has been used to develop new visual stimuli, and brief videos are also being applied. Drawings, which may have lower validity, were often used for assessment in children. Research that uses functional and structural MRI or EEG as methodological alternatives has increased in the last decade. This is a rapidly changing field, and more studies are needed to compare methodologies that are used to explore impairments in facial emotion recognition.