No abstract

A sulfidogenic bioreactor, operated at low pH (4–5), was set up and used to remove transition metals (copper, nickel, cobalt, and zinc) from a synthetic mine water, based on the chemistry of a moderately acidic (pH 5) drainage stream at an operating copper mine in Brazil. The module was constructed as an upflow biofilm reactor, with microorganisms immobilized on porous glass beads, and was operated continuously for 462 days, during which time the 2 L bioreactor processed >2,000 L of synthetic mine water. The initial treatment involved removing copper (the most abundant metal present) off-line in a stream of H2S-containing gas generated by the bioreactor, which caused the synthetic mine water pH to fall to 2.1. The copper-free water was then amended with glycerol (the principal electron donor), yeast extract and basal salts, and pumped directly into the bioreactor where the other three transition metals were precipitated (also as sulfides), concurrent with increased solution pH. Although some acetate was generated, most of the glycerol fed to the bioreactor was oxidized to carbon dioxide, and was coupled to the reduction of sulfate to hydrogen sulfide. No archaea or eukaryotes were detected in the bioreactor microbial community, which was dominated by acidophilic sulfate-reducing Firmicutes (Peptococcaceae strain CEB3 and Desulfosporosinus acididurans); facultatively anaerobic non-sulfidogens (Acidithiobacillus ferrooxidans and Actinobacterium strain AR3) were also found in small relative abundance. This work demonstrated how a single low pH sulfidogenic bioreactor can be used to remediate a metal-rich mine water, and to facilitate the recovery (and therefore recycling) of target metals. The system was robust, and was operated empirically by means of pH control. Comparison of costs of the main consumables (glycerol and yeast extract) and the values of the metals recovered showed a major excess of the latter, supporting the view that sulfidogenic biotechnology can have significant economic as well as environmental advantages over current approaches used to remediate mine waters which produce secondary toxic wastes and fail to recover valuable metals.

Using acidophilic bacteria to catalyze the reductive dissolution of oxidized minerals is an innovative process that facilitates the extraction of valuable base metals (principally cobalt and nickel) from limonites, which are otherwise often regarded as waste products of laterite mining. The most appropriate conditions required to optimize reductive mineral dissolution are unresolved, and the current work has reassessed the roles of Acidithiobacillus spp. in this process and identified novel facets. Aerobic bio-oxidation of zero-valent sulfur (ZVS) can generate sufficient acidity to counterbalance that consumed by the dissolution of oxidized iron and manganese minerals but precludes the development of low redox potentials that accelerate the reductive process, and although anaerobic oxidation of sulfur by iron-reducing species can achieve this, less acid is generated. Limited reduction of soluble iron (III) occurs in pure cultures of Acidithiobacillus spp. (Acidithiobacillus thiooxidans and Acidithiobacillus caldus) that do not grow by iron respiration. This phenomenon (“latent iron reduction”) was observed in aerated cultures and bioreactors and was independent of electron donor used (ZVS or hydrogen). Sufficient ferrous iron was generated in the presence of sterilized hydrophilic sulfur (bio-ZVS) to promote the effective reductive dissolution of Mn (IV) minerals in limonite and the solubilization of cobalt in the absence of viable acidophiles.

The abundance of limonitic laterite ores in tropical and sub-tropical areas represents a large, and mostly unexploited, cobalt resource. Bioprocessing oxidised ores, and also waste materials such as tailings and processing residues, using acidophilic microorganisms to catalyse the reductive dissolution of iron and manganese minerals, is an environmentally benign alternative approach of extracting valuable base metals associated with these deposits. This work describes results from laboratory-scale experiments in which five cobaltbearing materials, three primary limonitic laterite ores and two processing residues (filter dust and slag), all sourced from mines and a processing plant in Greece, were bioleached under reducing conditions by a consortium of acidophilic bacteria (using elemental sulfur as electron donor) in stirred tank bioreactors at pH 1.5 and 35ºC. Whilst the target metal, cobalt, was successfully bioleached from all five materials (40 -50% within 30 days) the extraction of some other metals was more variable (e.g. between 2 and 48% of iron). Concentrations of soluble cobalt were highly correlated, in most cases, with those of manganese, correlating with the finding that cobalt was primarily deported in manganese (IV) minerals. Acid consumption also differed greatly between mineral samples, ranging between 3 and 67 moles H 2 SO 4 g -1 cobalt extracted. Comprehensive mineralogical analysis of the three limonitic samples before and after bioprocessing revealed significant variations between the ores, and demonstrated that elemental and mineralogical variabilities can greatly impact their amenability for reductive bioleaching.

Rosuvastatin calcium (ROS), ( Figure 1 ) belongs to the "statins" group, which is the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor. This drug is indicated for dyslipidemias treatment and can help to decrease the level of "bad cholesterol" and can consequently reduce the development of atherosclerosis and the risk of heart diseases. ROS was developed by Astra-Zeneca and it was approved in 2003 by the FDA in the United States. In 2015, under the trade name Crestor®, it was the fourth largest selling drug in the United States with sales above $5 billion. This study presents a literature review of analytical methods for the quantification of ROS in pharmaceutical preparations and biological fluids. The major analytical methods described in this study for ROS were spectrophotometry, high-performance liquid chromatography (HPLC) coupled to ultraviolet (UV) detection, and tandem mass spectrometry (LC-MS/MS).