This research communication depicts the quality and authenticity of grated Parmesan cheese sold in Brazil, with novel emphasis on ripening time. The sampling included all brands manufactured and packed at Brazilian dairy plants under federal inspection and sold in Londrina between June and September 2017. A total of 24 samples were analyzed (3 batches of 8 brands) for microbiological and compositional characteristics, including urea-PAGE electrophoresis for the quantitative determination of the milk proteins. About 80% of the samples were insufficiently ripened and 32% with low-fat content. Faced with these economically motivated food frauds, the authenticity of grated Parmesan in Brazil may be under threat. It is high recommended that future surveys and regulatory agencies go further than microbiological assessment, including and emphasizing the ripening time as the main parameter of grated Parmesan cheese.

Considering the high prevalence of subclinical mastitis and its impacts on milk production, thematic studies are needed and can provide strategic data for its control. This study aimed at investigating the most frequent microorganisms associated with subclinical mastitis in dairy cows in the northwest State of Paraná, Brazil and its sensitivity to antibiotics. A total of 478 foremilk samples were tested. Streptococci were the most frequently genus observed (63%), followed by staphylococci (31%). Streptococcus mutans, S. sanguis, and S. uberis were the most frequently identified species. For Streptococcus spp., the antimicrobial resistance was higher for Ceftiofur, whereas for Staphylococcus spp. it was also possible to verify a high occurrence of resistance for Gentamicin + Amoxicillin. Our results strengthen the knowledge of the microbiological agent and antibiotic-resistance patterns of pathogens at regional level, targeting future strategies to reduce the environmental spread of pathogenic strains resistant to antibiotics.

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