Alterations of the transcriptome and proteome enable stress recovery, but coordination of these events under stress is only partly understood. Mathew et al. report that under stress, an RNA splicing complex disassembles and the splicing factor Hsh155 moves to protein aggregates, coinciding with a drop in splicing and concomitant repression of ribosome production.

Stress granules (SGs) are stress-induced membraneless condensates that store non-translating mRNA and stalled translation initiation complexes. While metazoan SGs are dynamic compartments where proteins can rapidly exchange with their surroundings, yeast SGs seem largely static. To gain a better understanding of the yeast SGs, we identified proteins that sediment after heat-shock by mass spectrometry. Proteins that sediment upon heat-shock are biased toward a subset of abundant proteins that are significantly enriched in intrinsically disordered regions (IDRs). Heat-induced SG localization of over 80 proteins were confirmed using microscopy, including 32 proteins not previously known to localize to SGs. We found that several IDRs were sufficient to mediate SG recruitment. Moreover, the dynamic exchange of IDRs can be observed via FRAP, while other components remain immobile. Lastly, we showed that the IDR of the Ubp3 deubiquitinase was critical for yeast SG formation. This work shows that IDRs can be sufficient for SG incorporation, can remain dynamic in vitrified SGs, and can play an important role in cellular compartmentalization upon stress.

This study examines the lived experiences of seven internationally diverse scholars from Canada, the United States, New Zealand, and Australia to answer the question: how do we make meaning of our collective boundary crossing experiences across disciplines and positions within SoTL? Our positions range from graduate student, faculty, and academic developers, to department chair and centre director. We conducted a phenomenological study, based on narratives of experience, and drew on Wenger-Trayner and Wenger-Trayner’s (2015) theoretical framework that explores the features of a landscape of practice. Guided by this framework, we analyze our boundary crossings and brokering across the “diverse, political and flat” features of the SoTL landscape. Our collective findings highlight the critical role brokers play in facilitating boundary crossings. Brokering is precarious, bringing people together, building trusting relationships, and developing legitimacy while negotiating deadlocks, bureaucracy, authorities, and a multitude of challenges. Brokers, we found, require strength and resilience to mobilise, influence, and drive change in the landscape to transform existing practices or create new ones. We suggest that our analytical process can be used as a tool of analysis for future research about how brokers influence the SoTL landscape of practice and how brokering enhances SoTL development, support, and leadership.

Equity, diversity, and inclusion are crucial to 21st-century higher education and are increasingly discussed, critiqued, and improved with multiple approaches to recognize intersectionalities and enact positive change in work, research, teaching, and learning (Byrd, Brunn-Bevel, & Ovink 2019).Undergraduate learners come to the classroom as a diverse mosaic with different cultures, talents, disciplinary backgrounds, orientations, lifestages, and classroom expectations. Instructors can deliberately design for equity, diversity, and inclusion, including for large first-year classes.Equity, diversity, and inclusion in teaching and learning are frequently discussed on the level of principle and theory, and it is important to translate work for all educators for implementation and practice, including teaching strategies useful to helping across disciplines (Hartwell et al., 2017). | Universal design for learning (UDL) can be implemented in classrooms to foster equity, diversity, and inclusionUniversal design for learning (UDL) is an educational framework that can help design classrooms that are inclusive of all students,

1The authors provide an in-depth proteomic study of yeast heat stress granule (SG) proteins. They 2 identified intrinsic disordered regions (IDRs) as one of the main features shared by these proteins and 3 demonstrated IDRs can be sufficient for SG recruitment. 4 3 Abstract 1Heat-stress triggers the formation of condensates known as stress granules (SGs), which store non-2 translating mRNA and stalled translation initiation complexes. To gain a better understanding of SGs, we 3 identified yeast proteins that sediment after heat-shock by mass spectrometry. Heat-regulated proteins 4 are biased toward a subset of abundant proteins that are significantly enriched in intrinsically disordered 5 regions (IDRs). SG localization of over 80 heat-regulated proteins was confirmed using microscopy, 6 including 32 proteins that were not known previously to localize to SGs. We find that several IDRs are 7 sufficient to mediate SG recruitment. Moreover, the diffusive exchange of IDRs within SGs, observed via 8 FRAP, can be highly dynamic while other components remain immobile. Lastly, we showed that the IDR 9 of the Ubp3 deubiquitinase is critical for SG formation. This work confirms that IDRs play an important 10 role in cellular compartmentalization upon stress, can be sufficient for SG incorporation, can remain 11 dynamic in vitrified SGs, and play a vital role during heat-stress. 12