Backgrounds: Bacteriocin has been used widely in industry as a biopreservative agent. The objective of the present study was to investigate the potency of Bacteriocin isolated from tiger prawn Penaeus monodon and freshwater shrimp Macrobrachium rosenbergii as an anti-bacterial on fish. Methods: A total of ten candidates of probiotic bacteria consisted of five isolates from tiger shrimps (H1, H2, H3, H4, H5) and five isolates from freshwater prawns (W1, W2, W3, W4, W5) were evaluated. Bacteriocin wasBacteriocin was produced by centrifugation at a speed of 150 rpm and at 37 °C for 24 hours. The bacteriocin extract was purified by adding sulphate ammonium salt {(NH4) 2SO4} at 80% of the saturation level. Bacteriocin activity was determined using a diffusion method against pathogenic bacteria Vibrio alginolyticus, Aeromonas hydrophillaAeromonas hydrophilla and Pseudomonas stutzeri. Bacteriocins were analyzed usinganalyzedusing High Performance Liquid Chromatography (HPLC) and Fourier Transform Infra-Red (FTIR). The data were subjected to analysis of variance (ANOVA) and followed with Duncans multiple range test. Results: Bacteriocins produced by bacteria isolate H4 from tiger prawn indicated the highest bacteriocin activity againstbacteriocin activity against Pseudomonas stutzeri at stutzeri at the diameter of inhibition zone of 887.10 ± 409.24 mm 2/mL. While isolate W2 from freshwater shrimp indicated inhibition zone of 1466.96 ± 127.62 mm 2/mL. Both bacteriocins were purified by chromatography column using Sephadex LH-20. Conclusion s: This finding showed that bacterial isolates H4 and W2 have the potential to produce bacteriocins which inhibit the pathogenic bacteria. FTIR analysis showed an amide group at wave number 1652cm -1 contained in the bacteriocins of isolates H4 and W2.
The biopore infiltration hole is a hole with a diameter of 10 cm - 30 cm with a depth of 30 cm - 100 cm which is added with organic waste and will help soil biota form a biopore hole so that it can trap the air flowing around it. Black Soldier Fly larvae are able to accelerate composting and ordering of pores in the biopore infiltration holes. Perumahan Putri Indah Kulim RT 02 RW 12 Kelurahan Mentangor rarely finds open land semisization and house widening which causes a reduction in the surface of ground air infiltration so that during the rainy season puddles easily occur and during the dry season it reduces groundwater reserves. This activity aims to provide knowledge and insight into the community about biopore infiltration holes by utilizing Black Soldier Fly larvae and inviting the public to apply this technology. This service activity begins with the socialization of the biopore infiltration holes, then introduces the tools and materials as well as direct practice in the field. The results of this activity had a positive influence on the community and many community members who wanted to apply the biopore infiltration hole technology using Black Soldier Fly larvae. This is the first step in creating a clean and healthy environment, especially in Putri Indah Kulim Housing RT 02 RW 12 Mentangor Village.
Banjir merupakan masalah yang hampir setiap tahun melanda wilayah perkotaan maupun pedesaan. Umumnya di perkotaan banjir lebih banyak disebabkan oleh tidak lancarnya aliran air (diselokan) akibat sampah yang dibuang ke aliran air dan berkurangnya kawasan resapan air. Kelurahan Delima Kecamatan Tampan terdapat kawasan perumahan sederhana yang memiliki halaman rumah terbatas berukuran sekitar 7m x13 m sampai dengan 10 m x 15 m per unit rumah. Untuk memenuhi kebutuhan air bersih, dikawasan ini menggunakan sumur bor yang lama-kelamaan akan mengurangi cadangan air bawah tanah. Kawasan perumahan tersebut kadang-kadang sudah disemenisasi, yang mengakibatkan kurangnya resapan air hujan sehingga rentan terjadinya banjir dan kekeringan. Untuk mengatasi hal ini, masyarakat dapat menerapkan teknologi lubang resapan biopori. Kegiatan pengabdian ini bertujuan untuk memperkenalkan teknologi lubang resapan biopori dan mengajak masyarakat untuk menerapkannya serta membuat biokompos dari lubang resapan tersebut. Metode kegiatan pengabdian ini dilakukan dengan teknik penyampaian materi dan diskusi serta demo atau praktek pembuatan lubang resapan biopori sebagai upaya penanggulangan banjir dan kekeringan di pemukiman sekitar Kelurahan Delima, Kecamatan Tampan, Pekanbaru. Dari hasil kegiatan ini diketahui bahwa masyarakat sasaran terlihat antusias terhadap materi yang disajikan dan terjadi penambahan pengetahuan serta wawasan peserta tentang teknologi resapan biopori sekitar 89,33%.
Diapers are made by cotton and pulp containing cellulose that can be used as a substrate in the production of cellulose enzymes. The purpose of this research is to determine the ability of cellulolytic fungi Trichoderma asperellum LBKURCC1 and cellulolytic bacteria S-22 to degrade the used diapers containing urine by solid fermentation for 10, 20, and 30 days. The activity of crude extract of cellulose enzyme was observed with CMC 2% as substrate at pH 5,5 (Trichoderma asperellum LBKURCC1) and pH 7 (bacteria S-22 isolate), incubation temperature 40ºC during 30 minutes by using Nelson Somogyi’s method. The result showed that used diapers can be used as substrate for cellulose enzyme production by fungi Trichoderma asperellum LBKURCC1 with the highest activity of cruide extract cellulose enzyme obtained at 10th days solid fermentation of (1,891 ± 1,453) × 10-3 U / mL and by bacteria S-22 isolate at 20th days solid fermentation of (2,854 ± 0,019) × 10-3 U/mL. It can be concluded that bacteria S-22 isolate would degrade the used diapers better than fungi Trichoderma asperellum LBKURCC1. The result of this research compared to quality standards compost of SNI: 19-7030-2004 have not fulfilled, so it can not be used as compost.
The cassava's production arm was quite abundant. Human ratings generally revolved around plantations and in rungs industries, much of which was said in cassava commodities. Most people consume cassava in the simple way of being boiled, fried, made in chips, and so on. In order not to dull the flavor of traditional foods but to be increasingly ogled by the styrofoampurvets, it is possible to make the products of the Singkong processed innovation into cassava frozen. The public's devoted activity is to helping people improve the economy, providing added insight and skill to processing cassava through cassava frozen and helping people form a group of tapioca women (KWT). The method used in this dedicated activity is by counseling between socializing and training and by demonstrations of Singkong frozen production. This activity involves women of family welfare development and housewives in three neighborhood associations is number 01, 02, and 03 at citizens association 12, Mentangor village districts,Tenayan Raya city districts. The result of this activity is that the target public seems enthusiastic about the material presented and has increased participants' knowledge and insight on assava frocenas are 100%.
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