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A moist fescue forage in round bales showed a heating process with two peaks of temperature. The first peak, reached after 8 h, was not related to the development of thermophilic fungi but was accompanied by the rapid elimination of the thermosensitive field flora. The second phase of heating took place during several weeks while thermophilic or thermotolerant storage fungi developed. Key words: mycoflora, hay, heating.

The rumen anaerobic fungus Caecomyces communis was grown in a fermentor in Lowe medium. We studied four polysaccharide hydrolases and three glycoside hydrolases at early and final stages. We found a difference in cell association for these enzymes depending on the developmental stage. The endocellulase and beta-D-fucosidase were early synthesized, and their activities decreased at the end of the developmental cycle. On the contrary, the beta-D-glucosidase, beta-D-xylosidase and xylanase activities increased during the cycle. The avicelase and the CM-cellulase activities linked with thalli increased, whereas the extracellular activities of these enzymes decreased.

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