This study evaluated the histological response and the expression of tenascin (TN) and fibronectin (FN) after pulp capping with mineral trioxide aggregate (MTA) or calcium hydroxide (CH). Class V cavities and pulp exposure were performed in 40 primary pig teeth. The pulps were capped with either MTA or CH, and the cavities were sealed with resin-modified glass ionomer cement. CH was used as a control. Seven and 70 days posttreatment, the animals were sacrificed and teeth were prepared for histological evaluation. TN and FN were detected by immunostaining. A severe inflammatory response was observed after 7 days in the CH group (p<0.043), while in the MTA group, a mild response was observed. Similar reparative dentin deposition was observed after 70 days for both groups (p<0.005). The expression of FN and TN was similar for both groups in the two periods evaluated. TN and FN were expressed during pulp reparative events, independently of the capping material.

The present study aimed to evaluate increasing levels of fish waste oil in laying hens diets on performance, egg quality, and sensory features of the eggs. 192 Hisex White laying hens with 29 weeks of age were used, with water and food ad libitum. The experimental design was completely randomized consisting of eight treatments corresponding to the inclusion levels of fish waste oil (0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 and 3.5%) in the diets, with four replicates of six birds each. Data collected were subjected to polynomial regression at 5% of significance. Differences (p<0.05) were observed in feed intake and egg mass. Feed intake increased until 2.50% of fish waste oil in the diets. Differences were not observed (p>0.05) in all variables analyzed. Differences were observed (p<0.05) in flavor. Eggs from birds fed diets up to 2.00% present better acceptance by the tasters. Above this level, there was a considerable drop in acceptance. From these results, the present study indicates that the use of fish waste oilin laying hens diets did not affect the egg quality. However, its high inclusion negatively affected the feed intake, egg mass, and egg flavor.

The objective of this study was to evaluate the effect of different hormonal protocols on the reproductive performance of Santa Inês ewes in Amazon environmental conditions. Twenty-two Santa Inês ewes between 3 and 4 years-old were distributed in a randomized block experimental design, where the treatments consisted of two protocols for estrus synchronization (short and long) with eleven animals each. Data on the occurrence of estrus were described for each protocol. Data of estrus, pregnancy, and prolificity were firstly subjected to ANOVA and a subsequent Tukey’s test. Results were considered significant at p ≤ 0.05. The short-term protocol presented an interesting successful rate, where above 70% ewes tested had estrus. The long-term protocol also achieved a high successful rate, where above 80% ewes tested had estrus. However, comparing the protocols, the long-term protocol presented better results of positive estrus and pregnancy rates in ewes. Thus, it can be concluded that both protocols presented satisfactory results regarding estrus manifestation, and prolificity (lambs produced per ewe). However, under Amazon environmental conditions, the long-term protocol presented better results regarding positive manifestation of estrus and pregnancy rate.

RESUMO Objetivou-se com este trabalho avaliar a condição metabólica nutricional de ovelhas Santa Inês no período pré-parto e pós-parto, a fim de evitar a toxemia. Foram utilizadas 50 ovelhas adultas da raça Santa Inês criadas sobre a mesma condição de manejo e alimentação, distribuídas em cinco grupos, de acordo com o período de gestação: T -60 (60 dias antes do parto), T-30 (30 a 21 dias antes do parto), T0 (no parto até 4 dias após o parto), T+30 (30 dias após parto) e T+60 (60 dias após o parto). A coleta de sangue foi conduzida pela manhã por venopunção da jugular em dois tubos a vácuo. Foram avaliados os seguintes metabólitos: protéico (proteína total, albumina, globulina e uréia), energético (glicose e ß-hidroxibutirato) e mineral (magnésio (Mg), cálcio (Ca) e fósforo (P)). Para determinar as diferenças entre as médias foi aplicado o teste Tukey a 5% de probabilidade. As concentrações séricas do metabolismo protéico (proteínas totais, albumina, globulinas), mineral (Mg, Ca e P) e energético (glicose) em função das fases experimentais apresentaram diferenças significativas (P<0,05), onde as médias de β-hidroxibutirato mantiveram-se dentro dos valores de referência para a raça. Nenhuma das fases de gestação apresentou deficiências metabólicas nos aportes proteico e energético em função da dieta e da fase de manejo testada. O mineral cálcio apresentou níveis diminutos ao final da gestação e no inicio da lactação.

O objetivo deste estudo foi avaliar o potencial de produção de silagem a partir do reaproveitamento dos resíduos provenientes do beneficiamento do tambaqui. Os tratamentos se diferenciaram quanto à quantidade de inóculo (culturas puras da bactéria Lactobacillus plantarum ATCC 8014), sendo de 2,5%; 5,0%; e 7,5% (v/p). O período de inoculação da silagem foi de 35 dias. Os parâmetros de pH foram T1 - 4,81±0,04; T2 - 4,91±0,06; e T3 - 4,85±0,03; para acidez titulável os percentuais de T1 - 3,43±0,03; T2 - 3,43±0,05; e T3 -3,51±0,04. Durante a avaliação centesimal da silagem biológica úmida do resíduo de tambaqui os tratamentos mostraram-se sem diferença significativa e se mantiveram próximos em relação ao resíduo in natura, enquanto que na silagem biológica desidratada as características de rendimento e físico-químicas da silagem em todos os tratamentos apresentaram resultados expressivos e de alta qualidade com PB em T1 - 32,49±0,54; T2 - 33,65±1,26; e T3 - 37,15±1,0; teor lipídico em T1 - 30,44±0,09; T2 - 36,44±4,35; e T3 - 33,92±3,65; cinzas em T1 - 15,44±0,23; T2 - 16,46±0,29; e T3 - 15,67±0,37. O desenvolvimento da silagem de pescado biológico torna-se uma alternativa para a indústria pesqueira, promovendo um destino sustentável e lucrativo aos resíduos gerados durante o processamento, constituindo uma opção de matéria-prima de qualidade para o desenvolvimento de produtos com alto valor agregado de nutrientes, com potencial para utilização na alimentação de animal.