Animals corpses in teaching and research institutions could be sources of infection for students and teachers when applied for dissection and surgical practice. This research aimed to evaluate cats' corpses' conservation using a new anatomic technique and vacuum package for seven days, aiming surgical practicing. A 150 mL/ kg of alcohol with 5% glycerin and 120 mL/kg of a 20% sodium chloride, 1% nitrite, and 1% sodium nitrate solution was injected on corpses sealed in vacuum packages and put on 0 to 4°C. Skin and jejunum were collected on day 0 (fresh samples/control), and traction analysis was performed for seven consecutive days. On the last day, the liquid in the plastic bags was microbiologically analyzed. There was no statistical difference between control and conservation moments (D1 and D2) in maximal rupture force of the skin, and jejunum was similar to control in D2, D4, and D6. The microbial population did not exceed 6.0x10 4 CFU/mL in total aerobics and 4.8x10 4 CFU/mL in total anaerobes. Biomechanics was not signifi cantly affected, and the microbiological count was low during conservation, demonstrating the possible effectiveness of this anatomical technique for surgery training.
Summary The use of biological membranes in surgeries is a reality for years, and one of the most used is the bovine pericardium, so the purpose of this research is to describe the bovine pericardium's biomechanics by comparing two directions of a test, one parallel to the longitudinal heart axis and one perpendicular. 20 adult bovine pericardium were tested for the maximum rupture force and rupture elongation, collecting four samples of each pericardium direction. In phase 2, eight pericardia were conserved for 4 months in a 98% glycerine solution, and the solution in which they were submerged was microbiologically analysed monthly. The Mann–Whitney test was used; there was a very significant difference between the perpendicular and parallel groups (p = .0001). The T test showed no significant difference for the rupture elongation (p = .0938). In pericardium preserved in glycerine, the outliers were removed regarding the maximum rupture force, and a Boxcox transformation was performed (λ = 0.25). Outliers were removed for the rupture elongation, and Bartlett's test (p = .7836), and Cramer–Von Mises (p = .5033) were performed and then, the analysis of variance (p < .0001), followed by the Tukey test at 5%. In the microbiological analysis, there was no presence of microorganisms during conservation. The research has shown that the pericardium collection direction influences its resistance and it can be stored in glycerine for 4 months without losing biomechanical characteristics.
O presente trabalho traz uma revisão de literatura relacionada aos principais aspectos da lipidose hepática em aves adultas da espécie Amazona aestiva, conhecida como papagaio-verdadeiro ou papagaio-de-fronte-azul, cada vez mais popular como animal de companhia. A revisão trata sobre a fisiologia hepática e aspectos básicos da nutrição. O enfoque se dá em relação a etiopatogenia, diagnóstico e tratamento da doença do fígado gorduroso, afecção extremamente comum na clínica de aves, mas que ainda gera algum grau de dúvida para o médico veterinário. Isso porque a patogenia da doença, apesar de comum nas demais espécies domésticas, ainda é pouco elucidada em papagaios. Faz-se necessária então a busca por mais informações e estudos acerca dessa patologia, tão usual na espécie em questão. A literatura infelizmente ainda carece de dados precisos, o que torna o diagnóstico e tratamento dos pacientes acometidos um desafio.
El objetivo de este trabajo fue analizar biomecánicamente la piel de cadáveres de caninos preparados químicamente con alcohol etílico y sal de cura, y embalados al vacío, para la práctica da cirugía veterinaria, además de obtener la evaluación microbiológica que se puede presentar durante el proceso. Se trabajó con ocho cadáveres de caninos, de peso 7.96±1.48 kg. Los animales fueron inyectados con 120 ml/kg de una solución de cloruro de sodio al 20%, nitrito al 1% y nitrato de sodio al 1%, y 150 ml/kg de alcohol con glicerina al 5% y se mantuvieron en envases al vacío a tempretatura entre 0 y 4 °C. Se tomaron muestras de piel el día 0 (muestras frescas) y los días 30, 60, 90 y 120 para análisis biomecánico, así como para análisis microbiológico de los fluidos en los envases del embalaje plástico. La fuerza máxima de ruptura presentada por el grupo control y en los días de conservación demonstró que la fijación con las sales de cura y el almacenamiento en embalajes al vacío mantuvo las características biomecánicas de la piel hasta por 120 días en los cadáveres bajo refrigeración.
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