Biomechanics of the fresh and conserved bovine pericardium

Abstract

Summary The use of biological membranes in surgeries is a reality for years, and one of the most used is the bovine pericardium, so the purpose of this research is to describe the bovine pericardium's biomechanics by comparing two directions of a test, one parallel to the longitudinal heart axis and one perpendicular. 20 adult bovine pericardium were tested for the maximum rupture force and rupture elongation, collecting four samples of each pericardium direction. In phase 2, eight pericardia were conserved for 4 months in a 98% glycerine solution, and the solution in which they were submerged was microbiologically analysed monthly. The Mann–Whitney test was used; there was a very significant difference between the perpendicular and parallel groups (p = .0001). The T test showed no significant difference for the rupture elongation (p = .0938). In pericardium preserved in glycerine, the outliers were removed regarding the maximum rupture force, and a Boxcox transformation was performed (λ = 0.25). Outliers were removed for the rupture elongation, and Bartlett's test (p = .7836), and Cramer–Von Mises (p = .5033) were performed and then, the analysis of variance (p < .0001), followed by the Tukey test at 5%. In the microbiological analysis, there was no presence of microorganisms during conservation. The research has shown that the pericardium collection direction influences its resistance and it can be stored in glycerine for 4 months without losing biomechanical characteristics.

Use of rainbow trout skin treated with glutaraldehyde as a mesh for abdominal hernioplasty in rats

“…Infrared thermography is a valuable tool for capturing the radiation emitted by cellular movement in cutaneous tissue, immediate postoperative surgical trauma, and the body’s response to foreign bodies within biomaterials, which directly correlates with histological observations 10 , 26 . The documented increase in cellular activity, as indicated by higher temperatures on the side with the rainbow trout skin implant from the seventh day onwards, is attributed to cellular infiltrate within the implant and the initiation of angiogenesis 10 , 25 , 26 . Similarly, this trend aligns with observations in later periods, where the implant begins the incorporation process, as evidenced by histological findings.…”

“…After filleting, the skins were removed by friction with a knife and cleaned. Following, they were immersed in a 0.5% glutaraldehyde solution for 18 days 19 , 20 , 21 , rinsed with sterile 0.9% NaCl, and immersed in 92.8% alcohol for 10 minutes 24 , 25 . Finally, they were dried with sterile gauze and immersed in 100% glycerin for a minimum of 30 days 10 , 26 .…”

“…1b and c ), using a 4.0 nylon thread 10 , 26 , 27 . Skin closure was accomplished using an interrupted “Wolf” pattern with the same thread 25 , 26 .…”

Comparison of Bovine- and Porcine-Derived Decellularized Biomaterials: Promising Platforms for Tissue Engineering Applications

“…Stress–strain curves were gained depending on the measured strength, length, and thickness of the sample. The ultimate tensile strength (MPa), Young’s (elastic) modulus (MPa), and elongation at breakage (%) were measured [ 42 , 43 ].…”

Histomorphological evaluation of acellularized bovine pericardium in breast implant coverage

“…In its acellular form, it mimics the ECM of the host tissue, especially in the which refers to their three-dimensional organization and chemical composition. Furthermore, it presents itself as a material with wide availability and accessibility, which makes it a biomaterial with characteristics that favor wide use in the biomedical area (Soares et al, 2021;Braile-Sternieri et al, 2020;Mathapati et al, 2013;Goissis et al, 2011). The acellularization process is a procedure that removes cellular components from tissues without altering the integrity of the remaining ECM (Crapo et al, 2011), decreasing tissue antigenicity due to the elimination of cellular debris and soluble components.…”