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Fig. 1. Light micrograph of the B. ictericus integument. With the HE-staining the EK layer appears as basophilic dispersed material (arrows). The integument is composed of an epidermis (E) and a dermis, which is subdivided into the stratum spongiosum (SS) and the stratum compactum (SC). In the SS there are many dermal glands (+). BarsZ30 mm. Fig. 2. DMB-staining reveals a metachromatic reaction (arrows) in the EK layer. BarsZ45 mm.Fig. 3. The AB-staining at MgCl 2 0.05 M demonstrates positive reaction in the EK layer (arrow). The dermal gland also reveals positive reaction (+). EZepidermis. BarsZ30 mm.

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