Low-density polyethylene (LDPE) waste generates an environmental impact. To achieve the most suitable option for their degradation, it is necessary to implement chemical, physical and biological treatments, as well as combining procedures. Best treatment was prognosticated by Plackett-Burman Experimental Design (PB), evaluating five factors with two levels (0.25 mM or 1.0 gL-1 glucose, 1.0 or 2.0 mM CuSO4, 0.1 or 0.2 mM ABTS [2, 20-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)], pH 4.5 ± 0.2 or 7.0 ± 0.2 and 30 or 90 day incubation), which was reproduced for 150 days. Therefore, PB identified a sequential treatment (plasma followed by fungus) for partial LDPE biodeterioration. Sheets were pretreated with glow discharge plasma (O2, 3.0 x 10−2 mbar, 600 V, 6 min.), followed by Pleurotus ostreatus biodeterioration. Fungus growth, colonization percentage, and pigment generation followed. Laccase (Lac), manganese peroxidase (MnP) and lignin peroxidase (LiP) activities were appraised. Additionally, contact angle (CA), functional group presence and changes and carbonyl and vinyl indices (Fourier transformed infrared spectroscopy) were evaluated. LDPE surface changes were assessed by Young’s modulus, yield strength, scanning electronic microscopy (SEM), Fourier transformed infrared spectroscopy (FTIR) and atomic force microscopy (AFM). Plasma discharge increased hydrophilicity, decreasing CA by 76.57% and increasing surface roughness by 99.81%. P. ostreatus colonization was 88.72% in 150 days in comparison with untreated LDPE (45.55%). After this treatment carbonyl groups (C = O), C = C insaturations, high hydrophilicity CA (16 ± 4) °, and low surface roughness (7 ± 2) nm were observed. However, the highest Lac and LiP activities were detected after 30 days (Lac: 2.817 U Lac g-1 and LiP: 70.755 U LiP g-1). In addition, highest MnP activity was observed at day 120 (1.097 U MnP g-1) only for P. ostreatus treated LDPE. Plasma favored P. ostreatus adsorption, adherence, growth and colonization (88.72%), as well as partial LDPE biodeterioration, supported by increased hydrophilicity and presence of specific functional chemical groups. The approximate 27% changes in LDPE physical properties support its biodeterioration.
The Hausdorff distance is a widely used tool to measure the distance between different sets. For the approximation of certain objects via stochastic search algorithms this distance is, however, of limited use as it punishes single outliers. As a remedy in the context of evolutionary multi-objective optimization (EMO), the averaged Hausdorff distance Δ p has been proposed that is better suited as an indicator for the performance assessment of EMO algorithms since such methods tend to generate outliers. Later on, the two-parameter indicator Δ p , q has been proposed for finite sets as an extension to Δ p which also averages distances, but which yields some desired metric properties. In this paper, we extend Δ p , q to a continuous function between general bounded subsets of finite measure inside a metric measure space. In particular, this extension applies to bounded subsets of R k endowed with the Euclidean metric, which is the natural context for EMO applications. We show that our extension preserves the nice metric properties of the finite case, and finally provide some useful numerical examples that arise in EMO.
The averaged Hausdorff distance ∆p is an inframetric which has been recently used in evolutionary multiobjective optimization (EMO). In this paper we introduce a new two-parameter performance indicator ∆p,q which generalizes ∆p as well as the standard Hausdorff distance. For p, q 1 the indicator ∆p,q (that we call the (p, q)-averaged distance) turns out to be a proper metric and preserves some of the ∆p advantages. We proof several properties of ∆p,q, and provide a comparison with ∆p and the standard Hausdorff distance. For simplicity we restrict ourselves to finite sets, which is the most common case, but our results can be extended to the continuous case.
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