The present study demonstrated Corynebacterium amycolatum as an emerging MDR nosocomial pathogen with emphasis on recognition of the class A β-lactamase encoding gene. Analysis of phenotypic and genotypic features of virulence mechanisms including C. elegans survival response to infection to C. amycolatum (imipenem - resistant and susceptible) strains were also investigated. Antimicrobial resistance genes were detected by polymerase chain reaction. We also evaluated the ability of Corynebacterium amycolatum in its ability to colonize and kill or host in a C. elegans infection model system and we investigated the adherence and invasion persistence of C. amycolatum within HEp-2 and U-937 cells. This study identified two C. amycolatum strains penicillin resistant and were positive for the blagene, encoding a class A betalactamase. The ability to C. amycolatum to kill the nematode C. elegans was verified. All strains of C. amycolatum caused a decline in the survival curve of C. elegans. The interaction of nematode and C. amycolatum results in morphological changes: Dar formation and “bag of worms” in all tested strains. About U-937 macrophages assay, the data indicate that the samples of Corynebacterium amycolatum studied had greater ability to adhere to the surface in the first 3 hours of infection of the monolayers (p <0.05). According to current knowledge, this is the first identification of C. amycolatumbla gene positive. So, we suggest that medical surveillance programs should include control strategies in order to decrease potential risk factors of nosocomial infections due to C. amycolatum.

Câncer de colo de útero está associado alguns subtipos do Papiloma Vírus Humano (HPV), sendo 100% tratável quando diagnosticado precocemente, pelo exame preventive e prevenido pela vacinação. Mesmo assim, este tipo de câncer está entre os casos de câncer mais incidentes. Este trabalho avaliou o nível de conhecimento sobre HPV, câncer, e vacinação na população de Nova Friburgo, para identificar as lacunas existentes no assunto que levam a população à falta de prevenção e conscientização pela desinformação e visão errônea dos temas abordados. Um questionário para análise do conhecimento com foi disponibilizado online, no site do projeto, para que a população respondesse. Foram coletados 290 questionários, as respostas foram analisadas e os números absolutos obtidos foram convertidos à percentuais para produção dos gráficos. Posteriormente, foram desenvolvidas políticas de conscientização e prevenção. Apesar da maioria dos participantes já terem ouvido falar sobre HPV, poucos sabem sobre as doenças associadas ao vírus e o risco de exposição à infecção. Há a necessidade da difusão de informações das complicações da infecção persistente e formas de prevenção. Os participantes buscam informações sobre saúde e bem-estar na internet, conclui-se que é de suma importância difundir informações verídicas e de qualidade neste veículo de comunicação.

The present study demonstrated Corynebacterium amycolatum as an emerging MDR nosocomial pathogen with emphasis on recognition of the class A β-lactamase encoding gene. Analysis of phenotypic and genotypic features of virulence mechanisms including C. elegans survival response to infection to C. amycolatum (imipenem - resistant and susceptible) strains were also investigated. Antimicrobial resistance genes were detected by polymerase chain reaction. We also evaluated the ability of Corynebacterium amycolatum in its ability to colonize and kill or host in a C. elegans infection model system and we investigated the adherence and invasion persistence of C. amycolatum within HEp-2 and U-937 cells. This study identified two C. amycolatum strains penicillin resistant and were positive for the blagene, encoding a class A betalactamase. The ability to C. amycolatum to kill the nematode C. elegans was verified. All strains of C. amycolatum caused a decline in the survival curve of C. elegans. The interaction of nematode and C. amycolatum results in morphological changes: Dar formation and “bag of worms” in all tested strains. About U-937 macrophages assay, the data indicate that the samples of Corynebacterium amycolatum studied had greater ability to adhere to the surface in the first 3 hours of infection of the monolayers (p <0.05). According to current knowledge, this is the first identification of C. amycolatumbla gene positive. So, we suggest that medical surveillance programs should include control strategies in order to decrease potential risk factors of nosocomial infections due to C. amycolatum.

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