The persistence of armed Muslim separatist rebellion in Southeast Asia is demonstrated by the ongoing rebellions in Aceh and Mindanao. A strong regional identity infused with Islam has been a binding factor in these separatist movements. Their persistence demonstrates the failure of Indonesia and the Philippines in achieving legitimacy for their post-independence political structures as well as continued internal weakness. The prospects for their quick and peaceful resolution are not good. The external dimension of Muslim separatism has heightened mistrust among states in the region and raised apprehensions over the broader issue of Islamic fundamentalism and the implications for the region should Aceh and Mindanao achieve secession.

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We present the nELISA, a miniaturised, high-throughput, and high-fidelity protein profiling platform. DNA oligonucleotides are used to pre-colocalize antibody pairs on spectrally encoded microparticles and perform displacement-mediated detection while ensuring spatial separation between non-cognate antibody pairs. Read-out is performed cost-efficiently and at high-throughput using flow cytometry. We assembled an inflammatory panel of 191 targets that were multiplexed without cross-reactivity or impact to performance vs 1-plex signals, with sensitivities as low as 0.1pg/mL and measurements across the platform spanning 8 orders of magnitude. We then performed a large-scale PBMC secretome screen, with cytokines as both perturbagens and read-outs, measuring 7,392 samples and generating ~1.5M protein datapoints in under a week, a significant advance in throughput compared to other highly multiplexed immunoassays. We uncovered 447 significant cytokine responses, including multiple putatively novel cytokine responses, that were conserved across donors and stimulation conditions. We also validated its use in phenotypic screening, and proposed applications for the nELISA in drug discovery.

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