Background. Balneotherapy has its rightful place in the arsenal of means of treatment of chronic. The universally recognized gold standard of balneofactors is Naftussya bioactive water. The issue of the loss of its healing properties by Naftussya water after extraction from the subsoil, the possibility of its preservation and transportation remain debatable and relevant. The purpose of this study is to compare the impact of fresh and canned water on the microbiota of feces and urine in patients with chronic pyelonephritis. Materials and Methods. The object of clinical-physiological observation were residents of the city of Truskavets’ (21 men aged 24-67 years and 8 women 33-76 years) with chronic pyelonephritis in remission. The subject of the study were the leukocyturia and bacteriuria levels and components of microbiota of feces. Results. Weekly use of fresh Naftussya water causes an increase in the reduced content of probiotics in the microbiota and a decrease in the increased content of conditionally pathogenic microflora, which is accompanied by a reduction of bacteriuria and leveling of leukocyturia. The beneficial effect on the listed parameters of canned Naftussya water is less pronounced, but the differences are statistically insignificant. Conclusion. The obtained results provide grounds for continuing research into the effects of canned Naftusya water on the body of urological patients.

Background. There are several patented methods of preservation of medicinal waters of the Naftussya type. The authors of the patents as evidence of the correctness of the methods cite the facts of preservation of the ability of canned water to enhance motility rat’s v. portae in vitro and/or increase diuresis. However, such tests are not attributive. The most characteristic manifestation of the biological activity of Naftussya water is the stimulation of phagocytosis. Another "Method of preservation of hydrogen sulfide mineral waters" was recently patented. Based on the above, we will use attributive tests for its verification. Materials and Methods. The object of clinical-physiological observation were residents of the city of Truskavets’ (21 men aged 24-67 years and 8 women 33-76 years) with chronic pyelonephritis in remission. The subject of the study were the phagocytic function of neutrophils, excretory function of kidneys as well as leukocyturia and bacteriuria levels and components of microbiota of feces. Results. Weekly use of fresh Naftussya water causes an increase in the reduced content of probiotics in the microbiota and a decrease in the increased content of conditionally pathogenic microflora, which is accompanied by an increase in reduced bactericidal capacity of neutrophils against both E. coli and Staph. aureus, normal diuresis, phosphaturia, magnesiumuria, ureauria while reduction of bacteriuria and leveling of leukocyturia as well as decrease in concentration of uric acid in urine and creatinine and cholesterol in plasma. The beneficial effect on the listed parameters of canned Naftussya water is less pronounced, but the differences are statistically insignificant. Conclusion. The obtained results confirm the effectiveness of the Dovzhenko© method for Naftusya water conservation and provide grounds for continuing research into the effects of canned Naftusya water on the body of urological patients.

Background. Drinking mineral water is one of the ethnopharmacologic factors. Organic substances, despite their presence in all drinking mineral waters, are still considered to be active only in the water of Naftussya type (which, due to mineralization less than 1 g/L, are not formally mineral), whereas the physiological activity of true mineral waters (which mineralization is greater than 1-2 g/L) are associated with electrolytes and trace elements. Previously, we identified the role of organic carbon and nitrogen of mineral waters in their effects on certain metabolic and neuro-endocrine parameters. The purpose of this study is to clarify the role of organic substances of same mineral waters in their effects on immunity parameters. Material and methods. Experiment was performed on 48 healthy female Wistar rats 240-290 g divided into 5 groups. Animals of the first group for 6 days administered a single tap water through the tube at a dose of 1,5 mL/100 g of body mass. In the second group we administered the water Naftussya from the Truskavets’ layer (Galychyna, Ukraine), in the third group the water Sophiya of the Truskavets’ field. The rats of the fourth group received the native water from the Hertsa (Bucovyna, Ukraine) field, and the last group received its artificial salt analogue. The day after the completion of the drinking course in all rats immunne parameters were registered. Results. On the basis of the correlation analysis with step-by-step exclusion, 9 immune parameters (Endotheliocytes of Thymus, Spleen Mass Index, Entropy of Splenocytogram, Natural Killers, B- and 0-Lymphocytes, Rod-shaped Neutrophils, Phagocytic Index and Microbian Count of Monocytes of Blood) are included in the regressive model for organic nitrogen (R=0,818) and 6 parameters (Endotheliocytes of Thymus, Fibroblastes and Reticulocytes of Spleen, Pan-Lymphocytes, Microbian Count of Neutrophils and Phagocytic Index of Monocytes of Blood) for organic carbon (R=0,690). Together, the organic components of the chemical composition of loading fluids determine their effect on the immune parameters by 77%. Conclusion. Organic substances of mineral waters play an essential role in their effects on the immune parameters of female rats.

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