The effect of furosemide on the development of the acute ischaemic renal failure in the dog was studied. 11 canine kidneys were used as controls (group I) and 12 as a group where furosemide (6–8 mg/kg of body weight) was given (group II) immediately after releasing the clamps. Urine volume and sodium clearance were found significantly higher in the second group of kidneys during a period of 60 min after restoration of the blood flow to the kidney. Urea clearances remained low with no noted difference between the 2 groups. By the end of the first hour osmolar and potassium clearances were found to be significantly higher in the second group. The above findings suggest that furosemide given after an induction of acute ischaemic renal failure in the dog provides, up to the 1st hour after recirculation, some benefit in water and solute excretion but no benefit in urea clearance.

BackgroundWe previously showed that dietary vitamin D3 (D3) at 50x the adequate intake (AI) increased functional capacity in the transgenic G93A mouse model of amyotrophic lateral sclerosis (ALS), with females exhibiting signs of D3 toxicity. ALS is a fatal neuromuscular disease characterized by death of motor neurons in the CNS, muscle weakness and paralysis.ObjectiveIn this pilot study, we analyzed the quadriceps of G93A mice following dietary D3 supplementation at 50x the AI for contractile proteins (α‐actin; troponin C, trop C), inflammation (IL‐6), cell stress response (Hsp70), mitochondrial efficiency (UCP3) and apoptosis (CASP3 cleaved/pro ratio), mechanisms contributing to ALS pathology.MethodsStarting at age 25 d, 41 G93A mice were provided food ad libitum with either adequate (AI; 1 IU D3/g feed; 12 M, 11 F) or high (HiD; 50 IU D3/g feed; 10 M, 8 F) D3. At age 113 d, the quadriceps were analyzed for protein content. Differences were considered significant at P ≤ 0.10.ResultsIn females, HiD had 87% higher CASP3 vs AI (P = 0.084). Males had 59% higher α‐actin (P = 0.063), 21% lower trop C (P = 0.017), 42% lower IL‐6 (P = 0.099) and 58% higher Hsp70 vs females (P = 0.097).ConclusionDietary D3 at 50x the AI increases apoptosis in the quadriceps of female G93A mice, which could be due to D3 toxicity. (Supported by NSERC and Faculty of Health‐York U).Grant Funding Source : Supported by NSERC and Faculty of Health‐York U

BackgroundWe previously demonstrated that dietary vitamin D3 (D3) restriction hastened the decline in functional outcomes following disease onset in the G93A mouse model of amyotrophic lateral sclerosis (ALS). ALS is a fatal neuromuscular disease characterized by motor neuron death, muscle weakness and progressive paralysis.ObjectiveIn this pilot study, we investigated the effects of dietary D3 at 2.5% the adequate intake (AI) in G93A mice on quadriceps contractile proteins (α‐actin; troponin C, trop C), inflammation (IL‐6), cell stress response (Hsp70), mitochondrial efficiency (UCP3) and apoptosis (CASP3 cleaved/pro ratio), mechanisms contributing to ALS pathology.MethodsStarting at age 25 d, 42 G93A mice were provided food ad libitum with either adequate (AI; 1 IU D3/g feed; 12 M, 11 F) or deficient (DEF; 0.025 IU D3/g feed; 10 M, 9 F) D3. Differences in quadriceps protein content were considered significant at P ≤ 0.10.ResultsIn males, DEF had 34% lower α‐actin (P = 0.096) and 38% higher trop C (P = 0.031) vs AI. In females, DEF had 32% lower trop C vs AI (P = 0.020). DEF males had 16% higher UCP3 (P = 0.093) and 57% higher trop C vs DEF females (P = 0.021).ConclusionDietary D3 restriction differentially alters markers of muscle contractile proteins in both sexes in G39A mice, which may explain the associated decline in functional capacity. (Supported by NSERC and Faculty of Health‐York U).Grant Funding Source : Supported by NSERC and Faculty of Health‐York U