Background: Digestive tract disorder known as Covid-19 is an infectious disease with a high level of morbidity and mortality because of the quick transmission. Prevention efforts are carried out by implementing health protocols, one of which is washing hands. Aim: to find out the difference in the behavior of Covid-19 transmission vigilance before and after being given 6-Step Hand Washing Health Education to Aisyiyah Members in the New Normal Era. Methods: This type of research is a Quasi Experiment with a time series design. The population is 31 people in the Aisyiyah area which was conducted in August 2020 with a total sampling technique. Results: Bivariate analysis showed that there were differences in the behavior of Covid-19 transmission vigilance before and after being given 6-Step Hand Washing Health Education to Aisyiyah Members in the New Normal Era with a P Value of 0.000. Significant differences were found between Handwashing Behavior Before being given health education and after being given health education p Value 0.00, hand washing behavior before being given health education and after being given health education p Value 0.000 and a month after being given Health Education Handwashing 6 Steps To members aisyiyah. These differences can be seen after statistical analysis using the Cochran test and then continued with the Post Hoc Mc test. Nemar to find out the significant difference between the three measurements. Conclusion: Health education has a difference in a person's behavior and requires observation more than once to maintain positive behavior. Keywords: Covid-19; hand washing; health education