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Objective. To access the effects of platelet-rich plasma (PRP) on the behaviour of human bone marrow-derived mesenchymal stem cells (hBMSCs), including proliferation and migration. Methods. PRP was diluted with DMEM/F12, resulting in concentrations of 1%, 2%, and 5%. The proliferation of hBMSCs was examined by 2 methods: cell-number counting with the haemocytometer method and the colony-forming unit-fibroblast (CFU-F) assay. Cell migration was evaluated using the scratch wound healing (SWH) assay; after that, the recorded digital images were analysed by the Image-Analysis J 1.51j8 software to compare the cell-free areas between groups after 0, 24, and 48 hours. Results. hBMSCs cultured in DMEM/F12 at PRP concentrations of 1%, 2%, and 5% were all able to proliferate and migrate. In the 5% PRP group, hBMSCs proliferated greatly with a significantly higher cell number than reported for all other groups on days 5, 7, and 9. CFU-Fs were observed in all groups, except for the negative control group. The SWH assay demonstrated that hBMSCs cultured in 2% and 5% PRP almost filled the artificial wound scratch and significantly migrated more than those of all other groups at both 24 h and 48 h. Conclusion. This study indicated that, due to the significant enhancement of cell proliferation and migration, 5% PRP might be the optimal concentration that should be used to promote the potential of hBMSCs in wound healing.

This study examines accrual earnings management in Vietnamese listed firms around equity issuances. Using a matching approach, we find that issuers tend to report higher earnings by aggressive recognition of discretionary current accruals before and during the years of equity offering. This results in significantly larger abnormal stock returns for the issuing firm-years, as the capital market overvalues firms with higher accrual earnings. However, regression results show that investors are subsequently disappointed by negative reversals in earnings, and suffer from significant negative abnormal returns in the third year after the issuance. These results provide additional empirical evidence in an emerging and transition market context, and caution investors against fixation on reported earnings in equity offerings.

Context Consumption of dietary macronutrients is associated with the progression of a wide range of inflammatory diseases, either by direct modulation of host immune response or via microbiome. This includes periodontitis, a disease affecting tooth-supporting tissues. Objective The aim of this work was to systematically review studies focusing on the effect of macronutrient (ie, carbohydrate, protein, fat) intake on periodontitis in rodents. Data Sources Electronic searches were performed in February 2021 using the PubMed and Web of Science databases. Out of 883 articles reviewed, 23 studies were selected for additional analysis. Data Extraction Investigators extracted relevant data, including author names; the year of publication; article title; macronutrient composition; number and species of animals and their age at the start of the experiment; intervention period; method of periodontitis induction; and primary and secondary periodontitis outcomes. Quality assessment was done using the risk-of-bias tool for animal studies. After completing the data extraction, descriptive statistical information was obtained. Data Analysis High intakes of dietary cholesterol, saturated fatty acids, and processed carbohydrates such as sucrose, and protein-deficient diets were positively associated with periodontitis in rodents. This included greater amounts of alveolar bone loss, more lesions on periodontal tissues, and dental plaque accumulation. In contrast, high doses of milk basic protein in diets and diets with a high ratio of ω-3 to ω-6 fatty acids were negatively associated with periodontitis in rodents. Conclusion This work highlights the fact that, despite the large body of evidence linking macronutrients with inflammation and ageing, overall there is little information on how dietary nutrients affect periodontitis in animal models. In addition, there is inconsistency in data due to differences in methodology, outcome measurement, and dietary formulation. More studies are needed to examine the effects of different dietary macronutrients on periodontitis and investigate the underlying biological mechanisms.

Abstract. The objective of this study is to verify the effects of 0.1% povidone-iodine (PVP-I) as subgingival irrigation on chronic periodontitis patients after 4, 6 and 8-week treatment. A clinical trial was conducted on 18 patients from 30 to 65 years old (mean age: 51.1±11.2) with moderate or severe chronic periodontitis at Faculty of Odonto-Stomatology at University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam 2017. Treatments of chronic periodontitis included oral hygiene instruction, scaling and root planing plus 0.1% PVP-I irrigation. Clinical parameters including plaque index (PI), gingival index (GI), bleeding on probing (BOP), pocket depth (PD), clinical attachment loss (CAL) and the bacteria level in subgingival plaque (BANA test) were evaluated at 4-time points: T0 (the initial visit), T4 (4 weeks after the initial visit), T6 (6 weeks after the initial visit) and T8 (8 weeks after the initial visit). The whole mouth (PI, GI, BOP, PD and CAL) parameters and BANA test score showed statistically significant reductions from T0 to T8 (p<0.001). The PD and CAL of moderately deep (PD ≥5mm and <7mm); and deep pockets (PD ≥ 7mm) were significantly decreased from T0 to T8 (p<0.001). The findings from the present study demonstrated that the topical application of 0.1% PVP-I, used as subgingival irrigation along with subgingival root debridement had beneficial influences on chronic periodontitis treatments.