Xanthomonadales comprises one of the largest phytopathogenic bacterial groups, and is currently classified within the gamma-proteobacteria. However, the phylogenetic placement of this group is not clearly resolved, and the results of different studies contradict one another. In this work, the evolutionary position of Xanthomonadales was determined by analyzing the presence of shared insertions and deletions (INDELs) in highly conserved proteins. Several distinctive insertions found in most of the members of the gamma-proteobacteria are absent in Xanthomonadales and groups such as Legionelalles, Chromatiales, Methylococcales, Thiotrichales and Cardiobacteriales. These INDELs were most likely introduced after the branching of Xanthomonadales from most of the gamma-proteobacteria and provide evidence for the phylogenetic placement of the early gamma-proteobacteria. Moreover, other proteins contain insertions exclusive to the Xanthomonadales order, confirming that this is a monophyletic group and provide important specific genetic markers. Thus, the data presented clearly support the Xanthomonadales group as an independent subdivision, and constitute one of the deepest branching lineage within the gamma-proteobacteria clade.

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Colored shade nets may affect plant growth and fruit yield of horticultural crops. The understanding of how colored shade nets influence plants, however, is far from complete. The objective of this study was to determine the effects of colored shade nets on bell pepper fruit yield, postharvest transpiration, color, chemical composition, and antioxidant capacity. The experiment was conducted in Tifton, GA, during the spring of 2015 and 2016. The experimental design was a randomized complete block with four replications and five colored shade net treatments (black, red, silver, and white nets, and an unshaded control). The nets were placed on the top of wooden rectangular structures (15 m wide × 6 m long × 5 m high), leaving the sides of the structures uncovered. Results showed that in both 2015 and 2016, marketable and total fruit number, yield, and individual fruit weight were reduced under the unshaded treatment. There were inconsistent differences in marketable and total fruit number, yield, and individual fruit weight among colored shade nets. Postharvest fruit transpiration and skin permeance were also reduced in unshaded conditions, and no differences were found among colored shade nets. Fruit color L* and b* values were highest, and a* value was lowest in unshaded conditions. Fruit soluble solids, total phenols, flavonoids, and antioxidant capacity [Cupric Reducing Antioxidant Capacity (CUPRAC) and Trolox Equivalent Antioxidant Capacity (TEAC)] responded differently among colored shade nets in the 2 years. Total phenols, flavonoids, and TEAC, however, were among the highest in unshaded conditions. In conclusion, results of the present study support previous findings that shade nets increase fruit yield and quality in bell pepper compared with fruit produced in unshaded conditions. Nevertheless, there were no consistent differences in fruit total and marketable yield and postharvest fruit transpiration and chemical composition of fruit produced under colored shade nets.