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The productivity of crops is highly affected by the seedling quality, which is governed by seeding density in the nursery. So, an experiment was conducted to explore the effect of seedling spacing in the growth attributes of Broad Leaf Mustard cv. “Marpha Chauda Paate” at IAAS, Lamjung Campus, during Oct 2018. Four spacing treatments viz 0.5 cm × 1 cm, 1 cm × 1 cm, 1.5 cm ×1.5 cm and 2 cm × 2 cm were arranged in RCB Design with 5 replications. Observation of seedling height, leaf area, leaf number, shoot & root fresh weights, shoot dry matter, and dry matter percentage from twenty-three days old seedlings were recorded.  The total leaf area was estimated using the Image-J package. Data were tabulated in MS Excel and analyzed by Gen Stat. Treatments differed significantly in seedling height, shoot and root fresh weight, leaf area, root length, and shoot dry weight, while the number of leaves and dry matter percentage did not differ statistically. Maximum shoot fresh weight (1.09 g), shoot dry weight (0.11 g), leaf area (48.24 cm2), root length (4.89 cm), root fresh weight (0.03 g) per plant and shoot dry matter percentage (9.24%) were found in widest spacing (2 cm × 2 cm). However, seedling height was recorded higher in closer spacing. Therefore, the study of the overall characteristics asserted that the seed spaced at 2 cm × 2 cm produced superior seedling over all other spacings. Int. J. Appl. Sci. Biotechnol. Vol 8(1): 65-70

More travellers are making swift ascents to higher altitudes without sufficient acclimatization or pharmaceutical prophylaxis as road connectivity develops in the Himalayan region of Nepal. Our study connects ascent rate with prevalence and severity of acute mountain sickness (AMS) among patients admitted to the emergency ward of the Mustang district hospital in Nepal. A register-based, cross-sectional study was conducted between June 2018 and June 2019 to explore associations of Lake Louise scores with ascent profile, sociodemographic characteristics, and comorbidities using chi-square test, t-test, and Bayesian logistic regression. Of 105 patients, incidence of AMS was 74%, of which 61%, 36%, and 3% were mild, moderate, and severe cases, respectively. In the Bayesian-ordered logistic model of AMS severity, ascent rate (odds ratio 3.13) and smoking (odds ratio 0.16) were significant at a 99% credible interval. Based on the model-derived counterfactual, the risk of developing moderate or severe AMS for a middle-aged, non-smoking male traveling from Pokhara to Muktinath (2978m altitude gain) in a single day is twice that of making the ascent in three days. Ascent rate was strongly associated with the likelihood of developing severe AMS among travellers with AMS symptoms visiting Mustang Hospital’s Emergency Ward.