The aim of this study was to compare the visibility threshold of eight plates with different chromatic contrast. The staircase psychophysics method based on the resolution of gaps in Landolt C was used to determine the average visibility threshold. Thirty young adults with best-corrected visual acuity of 6/6, normal colour perception and no history of ocular diseases were recruited. The results showed a combination of white on blue background plate gave a highest visibility level (mean=44.48±6.37m), while red on a blue background was the least visible combination (mean=33.30±4.68m). In conclusion, the chromatic contrast of an object can affect the visibility threshold. Keywords: chromatic contrast, visibility threshold