Online learning is the key to the implementation of learning in the Covid 19 pandemic and the New Normal era. The purpose of this study was to determine the effectiveness of the e-learning course on subjects ‘Learn and Learning’ with Moodle-based for prospective teachers in Indonesia. This research was conducted using the Berg and Gail development method. The development instruments were validated by experts, and further product development was carried out in an integrated and simultaneous manner by the Higher Education Research Consortium Team (KRUPT) from Padang State University, Malang State University, Jakarta State University, Medan State University and Surabaya State University. This product is declared valid by the Expert, both in content, design and IT. Tests were limited during the Covid 19 pandemic and data collection was carried out online. Data collected in the form of an online questionnaire to student respondents (n = 40). Based on respondent questionnaire data, this product was declared to be very suitable for online learning, reaching 3.967 which indicated the highly acceptance level. Based on this research, it was concluded that e-learning products for subjects with learning and learning subjects could be widely used in the Educational Personnel Education Institution in Indonesia.

Background Dental caries is a widespread disease that causes dental tissue destruction and leads to local and general complications. Gram-positive bacteria including Streptococcus mutans , Streptococcus sanguinis , and Enterococcus faecalis take part in dental caries formation. Gram-positive bacteria have cell walls that consistof a thick layer of peptidoglycan which maintains the strength and rigidity of the bacteria, as well as bacteria guard from internal osmotic pressure. The biosynthesis of peptidoglycan involves many enzymes, including the Mur family, penicillin binding protein (PBP), and sortases. Purpose This research has the intention to screen and examine the antibacterial compound of edible plant Kemangi ( Ocimum basilicum L.) in terms of how it fights against some oral pathogenic bacteria of E. faecalis ATCC 29212, S. mutans ATCC 25175, and S. sanguinis ATCC 10566. Materials and Methods The O. basilicum L. was macerated by several organic solvents to obtain the extracts, before then being purified using several combinations of chromatography methods and the compound was discovered via spectroscopic methods. For the assay against bacteria, the extracts and compounds were tested using agar well diffusion and microdilution assay. Results The isolated compound was identified as β-sitosterol. The compound activity against bacteria was evaluated by in vitro assay against S. sanguinis ATCC 10566 and E. faecalis ATCC 29212 with the MIC and MBC value of 25,000 and 50,000 ppm, respectively. The compound was also tested by in silico study using the molecular docking method. The molecular interaction between β-sitosterol and the protein target showed a lower binding affinity value than the native ligand and other positive controls for each protein. Based on the amino acid residue bound to the ligands, β-sitosterol on MurA and SrtA is not competitive to the positive control, showing potential as a natural antibacterial agent. Meanwhile, on the MurB and PBP, β-sitosterol and positive control do compete with each other. Conclusion The compound, isolated from O. basilicum L. leaf, was determined as β-sitosterol, which has the molecular formula C 29 H 50 O. The antibacterial activity of β-sitosterol by in vitro assay showed weak antibacterial activity, yet exhibited the potential to inhibit the biosynthesis of peptidoglycan and prevent bacteria cell wall formation by inhibiting MurA and SrtA activity via docking simulation.

Learning in the industrial revolution era 4.0 and the Covid 19 pandemic period shifted towards digital and online learning. This research aims to analyze the Basic Educational Course (MKDK) elearning product for Student Development that was developed. The method used is mixed method research, qualitative and quantitative mixture. The development of e-learning products uses the Borg and Gall method, while validity and practicality are carried out by questionnaire analysis to respondents online. The instruments for product development and testing have been declared valid by Expert, both in content, design and IT. Based on data from respondents (n = 40), MKDK e-learning products have a high acceptability (3.84) and are appropriate to use. From the aspect of e-learning that was examined, the material portion or content substance had the highest yield, 4,215. Meanwhile, the evaluation aspect received the lowest score, which was 3,588. Based on this research, it was concluded that e-learning products for subjects with learning and learning subjects could be widely used in the Educational Institution (LPTK) in Indonesia.

Learning in the Industrial Revolution Era 4.0 has shifted the role of learning teachers to confirm teachers. This research aims to analyze the acceptability of e-learning products in Educational Basic Courses (MKDK) of Education Professions for students at the Educational Institution (LPTK) in Indonesia. In this study conducted by the Borg and Gall development method, and the testing process is carried out online. Analysis of product feasibility using online questionnaires to 40 respondents. The result is an instrument of development declared valid by experts, both in substance material, learning design and information technology (IT). Based on the questionnaire score analysis, e-learning products have a high acceptance score, which is 3.88. This figure means that MKDK e-learning products in the Educational Profession have high feasibility, so they can be implemented for online lectures for LPTK students in Indonesia.