INTRODUCTION: Secondary infertility can cause some problems for the couples involved, particularly the women. It is supposed that the life style could influence the fertility status of the couples. This study was aimed to comparison the life style between secondary infertile and fertile women.MATERIALS AND METHODS: In this case-control study, 125 secondary infertile women as case group and 140 fertile women as control group were investigated in 2015. The subjects were selected from the fertility clinics and private office of the gynecologists in Zahedan, southeast of Iran. The data were collected using a structured questionnaire including reproductive history, socio-demographic variables, physical activity and Pittsburgh sleep quality. To analyze the data were used Chi-square and multiple logistic regression with Hosmer&Lemeshow method.RESULTS: By adjusting for potential confounders the secondary infertile women were more likely to have had higher BMI and be overweight (OR=2.89, 95%CI: 1.15-10.1) than fertile women. The sedentary and low sleep quality were significantly more common in infertile women than fertile women (OR=2.37, 95%CI: 1.005-9.7 and OR=2.92, 95% CI: 1.6-9.3, respectively). Also the chance of nutritional related disease among cases was more that controls (P<0.05). CONCLUSION: As life style related factors were associated with some reproductive variables in this study, thus it could be concluded that the life style and nutritional status not only influence the obstetric events, but directly effect on fertility via another paths. However, decreasing weight and increasing physical activity, improvement of sleep quality are all likely to decrease secondary infertility in women.