ABSTRAKPenelitian ini bertujuan untuk mengetahui keragaman gen hormon pertumbuhan (GH) dengan enzim MboII pada itik Sikumbang Janti dengan menggunakan penciri PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). Penelitian ini menggunakan sebanyak 50 sampel darah itik Sikumbang Janti. Sampel darah itik Sikumbang Janti diambil melalui vena achilaris sebanyak ± 1 ml. DNA sampel darah diisolasi menggunakan protocol Genomik DNA Purification Kit (Promega). DNA total diamplifikasi menggunakan sepasang primer F : 5'-CTG GAG CAG GCA GGA AAA TT-3' dan R: 5'-TCC AGG GAC AGT GAC TCA AC-3' yang menghasilkan fragmen exon 1 gen GH dengan panjang 801 bp. ABSTRACT This study aimed at determinating the gene diversity of growth hormone (GH) by the enzyme MboII identifier at ducks of Sikumbang Janti using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). This study used 50 blood samples of Sikumbang Janti ducks, taken through a vein achilaris as much as ± 1 ml. DNA blood samples were isolated using the protocol Genomic DNA Purification Kit (Promega). Total DNA was amplified using primer pair F: 5'-CTG CAG GCA GGA GAG AAA TT-3 'and R: 5'-TCC AGG TCA GAC GAC AGT AC-3' which resulted in exon
Penelitian tentang pertumbuhan bibit surian (Toona sinensis (Juss,) M. Roem) yang diinokulasi mikoriza pada media tanam tanah ultisol telah dilakukan dari bulan November 2014 sampai Februari 2015 di rumah kaca dan Laboratorium Fisiologi Tumbuhan Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas, Padang. Penelitian ini bertujuan untuk mengetahui pertumbuhan bibit surian yang diinokulasi dengan beberapa dosis Fungi Mikoriza Arbuskula (FMA) pada tanah ultisol. Penelitian ini menggunakan metode Rancangan Acak Lengkap dengan 4 perlakuan dan 6 ulangan. Adapun perlakuan yang diberikan adalah tanpa inokulasi; inokulan 5 g/tanaman; inokulan 10 g/tanaman; inokulan 15 g/tanaman. Hasil penelitian menunjukkan bahwa pemberian dosis FMA sebanyak 15 g/tanaman pada media tanah ultisol memperlihatkan pengaruh yang nyata pada pertambahan jumlah daun tetapi tidak berpengaruh terhadap pertambahan tinggi dan diameter batang serta berat kering tanaman selama 12 minggu pengamatan.
The Silokek Geopark area, Sijunjung Regency, is one of the tourist areas in West Sumatra Province which has a high level of biodiversity, which can be disrupted due to the presence of invasive plants. A study has been carried out which aims to determine the composition and structure of undergrowth in habitats invaded by weeds in the Silokek Geopark tourist area, Sijunjung Regency. This research was conducted from September to December 2022 using the quadratic method and plotting by purposive sampling with a plot size of 10 x 10 m with a sub-plot measuring 2 x 2 m. The composition of undergrowth in the habitat invaded by weeds found 24 families, 38 genera, 38 species, and 1049 individuals. Among the undergrowth found, there were 12 families, 20 genera, 20 species and 532 individuals of invasive plants with the dominant family being Asteraceae (30.41%), and the co-dominant family being Nephrolepidaceae and Poaceae with a percentage of 17 each. .83% and 11.63%. The highest important value index was in Nephrolepis biserrata (28.42%) and the lowest was in Musa acuminata (0.76%). The most dominant weed species was Mikania michranta with an IVI of 18.79%. The diversity index of the understorey plants in this area is categorized as high with a diversity index (H' = 3.24). Weed plant species are more successful in dominating open and disturbed areas with high levels of light intensity, gaps or wide gaps and forest edges can be utilized by invasive plant species to grow optimally.
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