Purpose: Bacterial biofilm develop on the surfaces of urinary catheter and proceed to cause full blown bacterial infections and sepsis. Urinary catheters, infection rates increase with the duration of catheterization at rates of per day with virtually all of those who undergo long-term catheterization becoming infected. Also antibiotics results in the adaptation and development of resistance leading to treatment failure, prolonged hospitalization, increased costs of care, and increased mortality. Methods: In the present study total 200 used urinary catheters were studied from the different hospitals of Amravati city in 2015-2016. Different bacterial uropathogens were isolated by conventional method and biofilm formation was studied by tissue culture plate (TCP). Antibiotic sensitivity was performed by disc diffusion method. Minimum inhibitory concentration (MIC) and Minimum biofilm eradicating concentration (MBEC) of triclosan was determined by TCP. Results: Out of total samples 93% are contaminated. Around 59% urinary catheters contain mixed consortia. Pseudomonas aeruginosa was found to be the strong biofilm forming and multidrug resistant organism. The most effective drug over seven bacteria isolates were chloramphenicol. Triclosan was used to test against the strong and moderate biofilm forming isolates the MIC of triclosan ranged between 1.5 and 1000 μg/ml and MBEC was between 800 and 3200 μg/ml Conclusions: From the study it was concluded that female are more prone to be infected with catheter associated infection. Pseudomonas aeruginosa was found to be deadly caused of infection, as it is highly resistant to antibiotics. Also triclosan showed effective result on the bacterial uropathogens.

Urinary tract infections (UTI) are very common in all the developed as well as developing countries in which the majority of infections are catheter associated. Catheter associated urinary tract infections (CAUTI) is one of the major causes of hospital acquired infections. The aim of this study is to investigate influential demographic factors responsible for contamination associated with the rate of CAUTI, while taking into account type of urinary catheter used, the most common organisms found, patient diagnosis, age, gender, and comparison with other studies. During the study, 22 uropathogenic species were isolated from the different segments of urinary catheter samples of the patients collected from 12 different hospitals of Amravati city,