The goal of this project was to isolate and identify individual components from aircraft de‐icing/anti‐icing fluids (ADAs) through a toxicity‐based bioassay analysis. A Microtox® bioassay fractionation scheme was used to isolate a number of active fractions from ADAFs. Active fractions were identified using multiple spectral techniques, including nuclear magnetic resonance, gas chromatography–mass spectrometry, liquid chromatography–mass spectrometry, and ultraviolet characterization. The primary Microtox‐active fraction was shown to be a mixture of benzotriazole and tolyltriazoles, which are used as corrosion inhibitors in ADAF formulations. The identity of the compounds was confirmed through spectral and Microtox‐toxicity analysis and comparison of commercially available standards.

Abstract-The goal of this project was to isolate and identify individual components from aircraft de-icing/anti-icing fluids (ADAFs) through a toxicity-based bioassay analysis. A Microtox bioassay fractionation scheme was used to isolate a number of active fractions from ADAFs. Active fractions were identified using multiple spectral techniques, including nuclear magnetic resonance, gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, and ultraviolet characterization. The primary Microtox-active fraction was shown to be a mixture of benzotriazole and tolyltriazoles, which are used as corrosion inhibitors in ADAF formulations. The identity of the compounds was confirmed through spectral and Microtox-toxicity analysis and comparison of commercially available standards.