No abstract

A three-step sequential self-assembly procedure was applied in preparing gold electrodes modified in a stable and controlled way by a monolayer of thiolated b-cyclodextrin (b-CD), with methylene blue (MB) included in its cavity as the active component of the monolayer, and octanethiol as the nonelectroactive spacer blocking the electrode surface not occupied by b-CD. MB acted as a mediator of electrons with respect to a solution soluble analyte, H 2 O 2 , and provided electrical contact between the electrode and solution resident enzyme, laccase, catalyzing reduction of oxygen to water.