2004
DOI: 10.1023/b:jiph.0000031135.42890.92
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Effects of Cyclodextrin Derivatives on the Catalytic Activity of Tyrosine Phenol-Lyase

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Cited by 14 publications
(11 citation statements)
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“…As concluded by other investigators, we suspected that the presence of HP-β-CD depleted the free substrate (due to the formation of the HP-β-CD-substrate complex) leading to the impairment in the catalytic rate of the enzyme. Such expectation has been in accord with the literature precedent that CD inhibits catalysis of other enzymes by sequestering their substrates and thus reducing the concentration of free substrates in solution [2931,45]. To probe whether or not the HP-β-CD mediated inhibition of MetAP was exclusively due to the sequestration/depletion of the enzyme’s substrate; we proceeded to determine the binding affinity of MetAMC substrate with HP-β-CD as well as determine the kinetic parameters of the MetAP catalyzed reaction under an identical experimental condition.…”
Section: Resultssupporting
confidence: 84%
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“…As concluded by other investigators, we suspected that the presence of HP-β-CD depleted the free substrate (due to the formation of the HP-β-CD-substrate complex) leading to the impairment in the catalytic rate of the enzyme. Such expectation has been in accord with the literature precedent that CD inhibits catalysis of other enzymes by sequestering their substrates and thus reducing the concentration of free substrates in solution [2931,45]. To probe whether or not the HP-β-CD mediated inhibition of MetAP was exclusively due to the sequestration/depletion of the enzyme’s substrate; we proceeded to determine the binding affinity of MetAMC substrate with HP-β-CD as well as determine the kinetic parameters of the MetAP catalyzed reaction under an identical experimental condition.…”
Section: Resultssupporting
confidence: 84%
“…Such variations involved (among others): (i) HP-β-CD— MetAMC complex not being converted to HP-β-CD—MetAMC—MetAP complex as found in the case of the CD dependent inhibition of polyphenol oxidases as well as other enzymes [45,49], (ii) HP-β-CD—MetAMC—MetAP serving as the productive complex, (iii) interaction of HP-β-CD with free enzyme (followed by formation of HP-β-CD—MetAMC—MetAP complex). To analyze the data of Figure 5 by the model of Scheme-3 vis a vis its variants, we employed Dynafit software.…”
Section: Resultsmentioning
confidence: 99%
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“…5,6 In our previous work, we studied the effect of the addition of permethylated cyclodextrins on the enzymatic reaction of tyrosine phenol-lyase. 7 For both permethylated a-and b-CDs, we have noticed the inhibition of the enzymatic reaction. On the other hand, the permethylated c-CD has caused the activation of tyrosine phenol-lyase.…”
Section: Introductionmentioning
confidence: 89%
“…2 Therefore, an increased interest in applications of CDs in enzymology has been observed in the past few years. [3][4][5][6][7][8] The CDs are used in enzyme studies as additives 3 (which alter the rate of the enzymatic reactions) and also as covalent modifiers of the enzymes 4 (the modified enzyme was more catalytically active and thermally stable than the native one). Another example of the applications of modified CDs in enzymology has been the co-lyophilization of CDs with the enzymes.…”
Section: Introductionmentioning
confidence: 99%