No abstract

The duraplasty is a standard procedure during neurosurgery for injuries and diseases of the brain. The hermetic closure of the dura mater is not always possible with the application of autologous tissues. Synthetic, allogeneic and xenogenic implants, which are currently used, have disadvantages, so the search for the material that would best meet the requirements for a dura mater scaffold continues. The purpose is to study the physical and chemical properties of the composite chitosan/polyethylene oxide (PEO) film and determine the effectiveness of its application for duraplasty in the experiment in vivo; to analyze its ability to biodegradation; to evaluate the effect of chitosan/PEO scaffold on the regeneration of dura matter.

Regeneration of the dura mater following duraplasty using a collagen film, a chitosan film, or a combination of both with gelatin, was studied in a craniotomy and penetrating brain injury model in rats. Collagen autofluorescence in the regenerated dura mater was evaluated using confocal microscopy with excitation at λem = 488 nm and λem = 543 nm. An increase in regeneration of the extracellular matrix of connective tissue and an increase in matrix fluorescence were detected at 6 weeks after duraplasty. The major contributors to dura mater regeneration were collagen films, chitosan plus gelatin-based films, and, to a much lesser extent, chitosan-based films. By using autofluorescence densitometry of extracellular matrix, the authors were able to quantify the degree of connective tissue regeneration in the dura mater following duraplasty.

Стаття містить рисунки, які відображаються в друкованій версії у відтінках сірого, в електронній-у кольорі.

Мета роботи. На основі аналізу даних спіральної комп'ютерної томографії (СКТ) та морфологічних досліджень обґрунтувати доцільність застосування нанокомпозитної металокераміки для здійснення відстроченої краніопластики у щурів в експерименті. Матеріали і методи. В експерименті у 10 білих щурів (маса тіла 300-450 г, середній вік 6-8 міс) виконана відстрочена краніопластика з використанням нанокомпозитної металокераміки.