Background:This study aimed to analyze the efficiency of three new microsatellite multiplex panels, which were designed to evaluate a total of 16 loci of the rice genome, based on single PCR reactions of each panel. A sample of 548 accessions of traditional upland rice landraces collected in Brazil in the last 25 years was genotyped, a database of allelic frequencies was established, estimates of genetic parameters were performed and analysis of genetic structure of the collection was developed.
-The objective of this work was to select and use microsatellite markers, to map genomic regions associated with the genetic control of thermosensitive genic male sterility (TGMS) in rice. An F 2 population, derived from the cross between fertile and TGMS indica lines, was used to construct a microsatellite-based genetic map of rice. The TGMS phenotype showed a continuous variation in the segregant population. A low level of segregation distortion was detected in the F 2 (14.65%), whose cause was found to be zygotic selection. There was no evidence suggesting a cause-effect relationship between zygotic selection and the control of TGMS in this cross. A linkage map comprising 1,213.3 cM was constructed based on the segregation data of the F 2 population. Ninety-five out of 116 microsatellite polymorphic markers were assembled into 11 linkage groups, with an average of 12.77 cM between two adjacent marker loci. The phenotypic and genotypic data allowed for the identification of three new quantitative trait loci (QTL) for thermosensitive genic male sterility in indica rice. Two of the QTL were mapped on chromosomes that, so far, have not been associated with the genetic control of the TGMS trait (chromosomes 1 and 12). The third QTL was mapped on chromosome 7, where a TGMS locus (tms2) has recently been mapped. Allelic tests will have to be developed, in order to clarify if the two regions are the same or not.Index terms: Oryza sativa, microsatellite, hybrid rice, genetic map. Mapeamento de controladores de caracteres quantitativos de macho-esterilidade gênica termossensível em arroz indicaResumo -O objetivo deste estudo foi selecionar e utilizar marcadores microssatélites, para mapear as regiões genômicas associadas ao controle genético de macho-esterilidade termossensível (TGMS) em arroz. Uma população F 2 , derivada do cruzamento entre linhagens indica fértil e TGMS, foi usada para construir um mapa genético de arroz, baseado em marcadores microssatélites. O fenótipo TGMS analisado apresentou uma variação contínua na população segregante. Um baixo nível de distorção da segregação foi detectado na população segregante (14,65%), e a seleção zigótica foi apontada como causa da distorção. Não houve evidência de relação de causa-efeito entre a seleção zigótica e o controle de TGMS nesse cruzamento. Um mapa de ligação com 1.213,3 cM foi construído, baseado nos dados de segregação da população F 2 . Noventa e cinco marcadores microssatélites, de um total de 116 marcadores polimórficos, foram reunidos em 11 grupos de ligação, com uma média de 12,77 cM entre os locos marcadores adjacentes. Os dados fenotípicos e genotípicos permitiram a identificação de três novos locos controladores de caracteres quantitativos (QTL) para a macho-esterilidade gênica termossensível em arroz indica. Dois dos QTLs foram mapeados em cromossomos que não tinham, ainda, sido associados ao controle genético da característica TGMS (cromossomos 1 e 12). O terceiro QTL foi mapeado no cromossomo 7, onde um loco TGMS (tms2) foi recentemente ...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.