Based on the hollow
fiber protected molecularly imprinted polymer,
a micro-solid-phase extraction (μ-SPE) method was developed
and applied for the analysis of indole-3-butyric acid in mung bean
sprouts by high-performance liquid chromatography. The extraction
conditions of the μ-SPE method were optimized using L9(34) orthogonal, and optimum conditions were found as
follows: pH of sample solution was 2.0, chloroform was the organic
solvent for embedding the μ-SPE bars, and acetonitrile was the
desorption solvent. In addition, the extraction time was 80 min, desorption
time was 5 min, stirring speed was 800 rpm, and concentration of NaCl
was 10%. Under the optimum conditions, a standard curve was established
for IBA, with a correlation coefficient of 0.9999. After extraction
with phosphate buffer solution (pH = 9.0), successful pretreatment
of mung bean sprouts was achieved by the μ-SPE method. The limit
of detection was 0.075 mg/kg, and the recoveries were found to be
in the range of 88.9–106.4%. This method is simple, environmentally
friendly, and can be used for the determination of indole auxin contents
in green bean sprouts quickly and accurately.
Polycyclic aromatic hydrocarbons (PAHs) will be ingested by people through different ways to threaten their health during play, so the environmental quality of the park directly affects the health of tourists and residents. Using eight typical parks in Urumqi in Northwest China as the study area, we used GC–MS to detect the PAHs content in the park surface soil and 10 common plants in the park in different seasons. The results showed that the content of PAHs in park soil in the summer was 5–6 times that in the winter, and the monomer PAHs in some park soil sampling points were higher than the soil pollution risk screening value. And the contamination level at these sampling sites was also higher compared to other sampling sites. In summer, the plants with high PAHs content in leaves are short herbs, while in winter, they are tall arbors. The PAHs of the park soil are mainly composed of high-cyclic aromatic hydrocarbons, and are mainly of traffic origin. The proportion of low-ring aromatic hydrocarbons in the winter was significantly higher than that in the summer. The source of PAHs in plants in summer is similar to that in soil, but the source of PAHs in plants in winter is more complex. The toxicity equivalent concentration method values of soil PAHs in South Park, Zhiwu Park, Shihua Park and Toutunhe Park were higher than that in other parks. The lifetime carcinogenic risk (ILCRs) values of some sampling points in these four parks in the summer were relatively high. The average ILCRs of adults and children in all parks reached a low-risk level in summer. The carcinogenic risk in children is much higher than that of adults.
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