a apri_julkarman@yahoo.co.id b ganjar@umm.ac.id c hariyadi@umm.ac.id *Corresponding author ARTICLE INFO ABSTRACT Keywords: Embryo development Epinephelus sp. Larval incubation Temperature Cantik grouper is the undue hybridization results grouper or cross-breeding between Epinephelus fuscoguttatus as a female and Epinephelus microdon as a male. The main barriers faced in the development of this commodity is still low levels of spawning up to seeding grouper. This study aimed to investigate optimum temperature observations against the rate of embryonic development Epinephelus sp. larvae. The fertilized eggs were incubated on 6 containers temperature treatment; each treatment there was repeated three times. The incubation temperature was kept on (A) 21-22 °C; (B) 23-24 °C; (C) 25-26 °C; (D) 27-28 °C; (E) 29-30 °C; (F) 31-32 °C. Results showed that eggs were incubated at a temperature of 21-22 ℃ embryonic development to a halt in the blastula, and temperature 23-24 °C stalled on phase myomere embryos. Temperature 25-26 °C needed 18 h 6 min by 8.33 % abnormality rate. Temperature 27-28 °C needed 16 h to hatch with a degree of abnormality of 7.6 %. Temperature 29-30 °C needed 15 h 1 min for the hatch to the degree of abnormality of 5.33 %. The 31-32 °C temperature needed 14 h 6 min to hatch with a degree of abnormality of 17.3 %. The limits of tolerance for the incubation of the eggs of Cantik grouper (Epinephelus spp.) were 26-32 °C. The best temperature of each treatment were obtained at a temperature of 29-30 °C. Based on our results, it concluded that the changing temperature affected how long eggs could hatch. How to cite: Simbolon AJ, Sutarjo GA, Hariyadi. 2019. The influence of difference temperature against the rate of embryonic development and larval cantik grouper abnormality (Epinephelus spp.). IJOTA. 2(1): 16-24.