About 2.8% of trauma sick persons hurt from peripheral nerve damages, thus, numerous approaches are using to improve peripheral nerve regeneration. In the current study, the efficacy of several dosages of Taurine for peripheral nerve regeneration was evaluated. About 0.1%, 1%, and 10% (w/w) of Taurine were added into thiolated chitosan hydrogel and its features including morphology, swelling properties, weight loss, hemo-, and cytocompatibility were assessed. Hydrogels’ functionality was evaluated by injecting them into the crushed sciatic nerve of rats by using walking-foot-print analysis, Hot plate latency test, gastrocnemius muscle wet weight loss, and histopathological evaluation. Results demonstrated that the average pore size is in the area of 30–40 μm with interconnected pores and their weight loss was around 70% after 7 days. Results of blood compatibility and the MTT tests confirmed the biocompatibility of hydrogels. In vivo study illustrate thiolated Chitosan/Taurine hydrogels especially hydrogel includes 1% of Taurine enhanced sciatic nerve regeneration. In conclusion, Taurine can be used as a feasible treatment for peripheral nerve regeneration.

The current study’s main aim was to fabricate and evaluate alginate (Alg) hydrogel containing retinoic acid (RA) as wound healing materials. Different RA concentrations (2, 10, and 50% w/w) were incorporated into the hydrogel. The results showed that the prepared hydrogels had a porous structure with a pore size of 69.69 ± 22.1 µm for pure Alg hydrogel and 78.44 ± 27.8 µm for Alg/RA hydrogel. The swelling measurement showed that the hydrogels swelled up to 65% and the incorporation of RA reduced the degree of swelling . The in vitro studies confirmed the hemo- and biocompatibility of the Alg/RA 2% and increasing the RA concentration induced hemolysis and toxic effects. The animal studies showed that the lowest RA concentration resulted in the best treatment outcome while increasing the RA concentration suppressed the healing process. In conclusion, these results showed that RA induced wound healing process at low concentration, and the prepared hydrogel could be used as the wound healing materials.