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The objective of this study was to evaluate the effects of the tithonia (Tithonia diversifolia) and elephant grass (Pennisetum purpureum) on in vitro digestibility and rumen fluid characteristics. This research was carried out using a randomized block design with 3 treatments (the level of tithonia and elephant grass) and 5 replications. The following treatments were performed: T1 = 100% tithonia; T2 = 100% elephant grass; T3 = 50% tithonia + 50 % elephant grass. The data were subjected to an analysis of variance (ANOVA), and differences between the treatment means were tested using Duncan’s Multiple Range Test (DMRT). The parameters measured were as follows: dry matter digestibility (DMD) (%), organic matter digestibility (OMD) (%), pH, volatile fatty acid (VFA) (mM) and NH3. The results revealed that DMD, OMD and VFA were significantly (P<0.01) increased by the 100 % tithonia (T1), pH were lowest (P<0.01) in T1. However, T1 and T3 non-significantly affected (P>0.05) NH3 Concentration. 100% tithonia resulted in the best in vitro digestibility and rumen fluid characteristics.

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