Auricularia auricula is an edible mushroom cultivated in Indonesia that has been known to have potential properties of bioactive compounds than can be used for medicinal purpose. This study aimed to examine the cytotoxic and apoptosis effect of chloroform extract of A. auricula on cervical cancer cells in vitro. The research design was in vitro experimental research. Cytotoxic tests was using an MTT [3-(4,5-dimetiltiazol-2-yl)-2.5-diphenyl tertrazolium bromide] assay and apoptosis test was using double staining method. Test of bioactive compounds was carried out using GCMS. Cytotoxic effect were analyzed by linear regression and apoptosis test was analyzed descriptively. Chloroform extract of mycelium A. auricula showed the best results with IC 50 = 264.87 μg/ml. An important finding obtained after the double staining process was that chloroform extract of A. auricula can induce HeLa cells death by apoptosis. GCMS test results showed that the extracts containing limonene and piperidinone which are the anticancer bioactive compounds. In conclusion, the chloroform extracts of A. auricula has the potential to inhibit the growth of cervical cancer cells. The benefit of this study are expected to provide information about the anticancer potential of extract of A. auricula against cervical cancer cells, thus contributing to the development of alternative anticancer treatments from natural product.

One of the biological agents in the biodegradation of organic waste is fungi. Some fungi have the Lignase enzyme, which can degrade Lignin (one of the main organic waste constituents) into simple sugars. The diversity and potential of organic waste degrading fungi still need much research to obtain potential types of fungi that can be developed in organic waste management. This study aimed to determine the diversity and potential of lignolytic fungi in the biodegradation of organic waste. The research was conducted using a descriptive method to take leaf and soil litter samples, isolate and purify fungi, and test lignolytic potential. Based on the isolation results from leaf litter samples, 16 types of fungal isolates were obtained. The lignolytic potential test was carried out using the Bavendamm method. In screening for potential lignolytic ability using the bavendamm method, 7 isolates were found to be positive. The highest lignolytic activity ratio was SR4BD isolate with a ratio value of 1.9. The results showed that the SR4BD isolate was Fusarium sp.

Sampah organik merupakan sampah yang tersusun dari senyawa organik. Penumpukan sampah organik cukup menjadi masalah serius. Jamur memiliki peran penting untuk mendegradasi sampah organik dalam proses pengomposan. Biodegradasi sampah organik berkaitan erat dengan kemampuan jamur dalam menghidrolisis senyawa amilum. Tujuan dari penelitian ini yaitu mengetahui isolat jamur pendegradasi amilum yang diperoleh dari sampah organik dan mengetahui potensi amilolitik dari isolat jamur tersebut. Penelitian ini dilakukan dengan metode survei dan eksperimental . Sampel sampah dapur yang terdiri atas sisa makanan dan bahan organik lainnya diambil dari rumah penduduk yang terletak di Kelurahan Bancarkembar, Bobosan, Grendeng, Karangwangkal, Pabuaran, Purwanegara, dan Sumampir. Tes screening menggunakan medium Starch Agar untuk mengetahui potensi amilolitik dari isolat jamur. Hasil menunjukkan terdapat delapan isolat jamur yang berpotensi dalam mendegradasi amilum. Sebanyak enam isolat jamur yang memiliki indeks amilolitik terbaik dengan nilai IE ≥ 1 teridentifikasi sebagai isolat Fusarium sp., Aspergilus sp., dan Penicillium sp. Selanjutnya untuk mengetahui aktivitas amilolitik secara kuantitatif dilakukan dengan metode DNS melalui pengukuran kadar glukosa. Isolat jamur Fusarium sp. memiliki aktivitas degradasi amilum yang paling tinggi dengan rata-rata kadar glukosa dari medium Fusarium sp. sebanyak 3.568,63 ppm

Composting is an alternative for recycling organic waste. Microorganisms that can degrade the components of organic waste are an essential part of composting. Isolation and selection of bacteria with the ability to degrade the elements of organic waste are the first steps to obtain an organic waste degrading agent. This study aimed to determine the characteristics and the enzymatic potential (cellulolytic, amylolytic, and proteolytic) of bacteria isolated from soil, cow dung and kitchen waste as candidates for organic waste degradation agents. The research begins with sampling and isolation of bacteria. The isolates obtained were tested qualitatively for their amylolytic, cellulolytic and proteolytic activities. Furthermore, bacterial isolates were characterized morphologically and biochemically. A total of 30 bacterial isolates were isolated from soil samples, cow dung and kitchen waste. The screening result showed that 6 isolates had amylolytic activity, 7 isolates had cellulolytic activity and 3 isolates had proteolytic activity. These bacterial isolates showed various morphological and biochemical characteristics. In general, soil samples, kitchen waste and cow dung contain potential bacteria as organic waste degrading agents

Coprinus comatus (O.F. Mull.) Pers. is a medicinal and edible mushroom containing bioactive compounds known as antidiabetic and antioxidant agents. The pancreatic β-cells were sensitive to reactive oxygen species (ROS) attacks, which can cause necrosis and disrupt insulin production. Therefore, this research aimed to evaluate the C. comatus ethyl acetate extract for antidiabetic and antioxidant to decrease fasting blood glucose, dipeptidyl peptidase-4 enzyme (DPP-4) levels, and increase glutathione peroxidase (GPx). Subsequently, 24 male Wistar rats were divided into six groups, namely 2-5 streptozotocin (STZ)-induced 45 mg/kg, 1/HC (without any treatment), 2/NC (negative control), 3/PC (administered metformin 45 mg/kg Body weight (BW), 4-6 (T1: administered 250 mg, T2: administered 500 mg, and T3: administered 750 mg/kg BW extract). The parameters were analyzed using ANOVA, followed by Duncan’s multiple range test. According to the results, the extract significantly decreased DPP-4 levels, blood glucose and increased GPx (p ≤ 0.05). The 500 mg extract effectively reduced blood glucose levels and DPP-4 enzyme as well as increased levels of GPx.