Chromium is one of the metals used in many areas of industry., However, chromium is toxic to organisms when present in large quantities in the environment. One of the method for treatment of hazardous waste containing chromium in the aquatic environment can be removed by bioremediation using sulfate-reducing bacteria (SRB). Therefore, the purpose of this research were to analyze the chromium precipitation activity of sulfate-reducing bacteria isolated from sulfate reducing bioreactor and its molecular identification using 16S rRNA gene sequences. The result observed that the isolate of sulfate-reducing bacteria (KGP1 strain) has chromium tolerancy ability up to 5 ppm. It also showed that the strain KGP1 could precipitate chromium up to 0.141 ppm (79 %) on 5 days incubation. Based on 16S rRNA gene sequences, this strain identified as Desulfovibrio aerotolerans.
Sampah organik merupakan sampah yang tersusun dari senyawa organik. Penumpukan sampah organik cukup menjadi masalah serius. Jamur memiliki peran penting untuk mendegradasi sampah organik dalam proses pengomposan. Biodegradasi sampah organik berkaitan erat dengan kemampuan jamur dalam menghidrolisis senyawa amilum. Tujuan dari penelitian ini yaitu mengetahui isolat jamur pendegradasi amilum yang diperoleh dari sampah organik dan mengetahui potensi amilolitik dari isolat jamur tersebut. Penelitian ini dilakukan dengan metode survei dan eksperimental . Sampel sampah dapur yang terdiri atas sisa makanan dan bahan organik lainnya diambil dari rumah penduduk yang terletak di Kelurahan Bancarkembar, Bobosan, Grendeng, Karangwangkal, Pabuaran, Purwanegara, dan Sumampir. Tes screening menggunakan medium Starch Agar untuk mengetahui potensi amilolitik dari isolat jamur. Hasil menunjukkan terdapat delapan isolat jamur yang berpotensi dalam mendegradasi amilum. Sebanyak enam isolat jamur yang memiliki indeks amilolitik terbaik dengan nilai IE ≥ 1 teridentifikasi sebagai isolat Fusarium sp., Aspergilus sp., dan Penicillium sp. Selanjutnya untuk mengetahui aktivitas amilolitik secara kuantitatif dilakukan dengan metode DNS melalui pengukuran kadar glukosa. Isolat jamur Fusarium sp. memiliki aktivitas degradasi amilum yang paling tinggi dengan rata-rata kadar glukosa dari medium Fusarium sp. sebanyak 3.568,63 ppm
Composting is an alternative for recycling organic waste. Microorganisms that can degrade the components of organic waste are an essential part of composting. Isolation and selection of bacteria with the ability to degrade the elements of organic waste are the first steps to obtain an organic waste degrading agent. This study aimed to determine the characteristics and the enzymatic potential (cellulolytic, amylolytic, and proteolytic) of bacteria isolated from soil, cow dung and kitchen waste as candidates for organic waste degradation agents. The research begins with sampling and isolation of bacteria. The isolates obtained were tested qualitatively for their amylolytic, cellulolytic and proteolytic activities. Furthermore, bacterial isolates were characterized morphologically and biochemically. A total of 30 bacterial isolates were isolated from soil samples, cow dung and kitchen waste. The screening result showed that 6 isolates had amylolytic activity, 7 isolates had cellulolytic activity and 3 isolates had proteolytic activity. These bacterial isolates showed various morphological and biochemical characteristics. In general, soil samples, kitchen waste and cow dung contain potential bacteria as organic waste degrading agents
One of the biological agents in the biodegradation of organic waste is fungi. Some fungi have the Lignase enzyme, which can degrade Lignin (one of the main organic waste constituents) into simple sugars. The diversity and potential of organic waste degrading fungi still need much research to obtain potential types of fungi that can be developed in organic waste management. This study aimed to determine the diversity and potential of lignolytic fungi in the biodegradation of organic waste. The research was conducted using a descriptive method to take leaf and soil litter samples, isolate and purify fungi, and test lignolytic potential. Based on the isolation results from leaf litter samples, 16 types of fungal isolates were obtained. The lignolytic potential test was carried out using the Bavendamm method. In screening for potential lignolytic ability using the bavendamm method, 7 isolates were found to be positive. The highest lignolytic activity ratio was SR4BD isolate with a ratio value of 1.9. The results showed that the SR4BD isolate was Fusarium sp.
Penelitian ini bertujuan untuk mengetahui efektivitas pemakaian zeolit alam dan arang dalam pengolahan air sumur sistem adsorpsi terhadap kualitas bakteriologis air ditinjau dari indeks MPN coliform dan colifekal. Selain itu juga, untuk mengetahui lama batas umur pemakaian maksimal zeolit alam dan arang dalam pengolahan air sumur sistem adsorpsi terhadap kualitas bakteriologis air. Penelitian ini dilakukan selama 3 bulan pada bulan Juni, Juli dan Agustus 2015 dengan pengecekan setiap bulan sekali dengan 3 kali ulangan. Sampel yang digunakan adalah air sumur sebelum dilewatkan dan sesudah dilewatkan pada alat penyaringan air sumur sistem adsorpsi menggunakan zeolit alam dan arang. Selanjutnya sampel air sumur tersebut dihitung nilai MPN coliform total dan colifekalnya dan dianalisis menggunakan metode deskriptif dan dikomparasikan dengan standar baku mutu air pada PERMENKES no. 416/ MENKES/ PER/ IX/ 1990. Selain itu, dilakukan penghitungan persentase efektivitas alat dalam menyaring bakteri untuk mengetahui batas pemakaian alat penyaringan air sistem adsorpsi. Hasil penelitian ini didapatkan data, nilai MPN coliform yang telah dilewatkan pada alat penyaring air sumur yaitu 34 dan 35 MPN/100 ml, sudah memenuhi kriteria baku mutu air yang ditetapkan yaitu berada ≤ 50/100 ml kecuali nilai MPN coliform air sumur pada bulan ke-0. Persentase efektivitas tertinggi alat penyaring sebesar 87,86% untuk penyaringan bakteri coliform total dan 85,20% untuk penyaringan bakteri colifekal. Persentase efektivitas alat menunjukkan kecenderungan penurunan setelah satu bulan pemakaian, sehingga batas umur pemakaian zeolit alam dan arang maksimal dalam alat penyaring air sumur sistem adsorpsi adalah satu bulan.Kata kunci: zeolit alam, arang, adsorpsi, bakteriologis air
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