Phenol is hazardous aromatic pollutant which needs to be treated to reduce its hazardous effects. Bioremediation using bacteria which can form biofi lm offer an alternative wastewater treatment that is cheaper and environmentally safe. Eighteen strains of phenol-degrading and biofi lm-forming bacteria were isolated from peat soil, also hospital and textile wastewater. Screening for phenol degradation ability of isolates were performed using Folin-ciocalteau reagent, while for biofi lm formation ability were performed using microtiter plate and crystal violet dye. Based on the ability to degrade phenol and to form biofi lm, four isolates (HP3, DOK135, DL120, andATA6) were choosen as phenol-degrading bacteria as well as biofi lm-forming bacteria. Based on phenotypic and genotypic characterization, isolate HP3 was highly similar to Rhodococcus equi strain DSM20307T, while DOK135 was highly similar to Enterobacter mori strain R18-2.The results also suggested that DL120 and ATA6 could be classifi ed to the genus of Micrococcus and Bacillus respectively.

<p>The aims of this study was to synthesize a compound of 3-methoxy-4-hydroxychalcone through Claisen-Schmidt condensation with grinding technique (solvent-free). Vanillin, acetophenone and 60 % NaOH catalyst used in this synthesis. Characterization of products was done with a spectrophotometer FTIR and ¹H-NMR spectrometer. The  product was obtained as a orange solid which has a melting point at    58 - 59 ˚C. Identification of the product by IR spectrophotometer showed the absorption of  C=C in the wavenumber of 1496.76 cm^-1 which confirmed that the hydration reaction of chalcone had occurred. Analysis using ¹H-NMR spectrometer also showed the proton of the CH=CH appearing on chemical shift (δ) 7.99 ppm. The antibacterial activity test showed that the compounds of 3-methoxy-4- hydroxychalcone has a potential as an antibacterial against bacteria <em>E. coli</em> and <em>B. subtilis</em>.</p>

Indonesia is a tropical country that has natural resources that are rich in biodiversity. One of these biodiversity is mushrooms. Mushrooms generally occupy various types of habitats, namely soil, wood, litter, animal waste and some grow on rotting mushrooms.. This study aims to study the diversity of Microscopic Fungi in the Cibereum Curug Route,Selabintana Resort, Gunung Gede Pangrango National Park, West Java. The research method used in this study is VES (Visual Encounter Survey) by combining transects following the Cibereum waterfall path, and taking the right and left areas of 1 meter. Data analysis was performed by qualitative descriptive analysis and quantitative descriptive analysis. The results of the research on the Curug Cibereum Resort Selabintana route, Gunung Gede Pangrango National Park (TNGGP) West Java, found as many as 30 species of basidiomycota mushrooms and 3 species from the ascomycota division. The most common species found in the study area were Coprinellus disseminatus (336 Number of individuals/4000 m2), Stereum sp (153 Number of individual/ 4000 m2), Mycena roseignicola (145 individual pieces/4000 m2), and Favolaschia pustulosa (138 Number of individual / 4000 m2). The diversity index of basidiomycota fungi in the Curug Cibereum route is in the medium category, namely H'= 1.004.