To analyse the serum cortisol and serum testosterone levels in idiopathic central serous chorioretinopathy. Material and methods: The prospective interventional study was conducted in the central laboratory associated with the Department of Ophthalmology, Subharti Medical College, Meerut. A study was conducted in 30 cases of CSR. The patients were divided into two groups i.e. Group A (15 patients with unilateral sudden painless loss of vision of less than one month of duration serving as cases and Group B (15 patients with no signs and symptoms serving as control). Evaluation of Macular thickness was done using OPTOVUE RTvue 100 OCT procedure. After pupil dilation, the patient is seated at the machine, asked to fixate at the internal fixation point and scan is obtained. The macular thickness map was taken to measure the thickness of macula. Fundus Fluorescein Angiography (FFA) was done, if required. Patients of both the groups underwent investigations such as dilated fundus examination, serum cortisol and serum testosterone levels. Results: Mean serum cortisol (μg/dL) among the case group was higher as compared to the control group with statistically significant difference as p<0.05. Mean serum cortisol levels compared between ink-blot pattern and smoke-stack patterns on FFA had no statistical significance. Mean serum testosterone (ngm/mL) among the case group (3.78±1.58) was lesser as compared to the control group (4.34±1.72) with statistically significant difference. Conclusion: ICSC is highly significant and significant associated with elevated 8.00 a.m. serum cortisol and testosterone level respectively.