No abstract

Biofilm formation helps the microbes to escape host defenses and develop resistance against antimicrobial agents. Early detection of slime production by the Candida species may direct the clinical management. The study was taken with the aim to estimate biofilm formation by Candida species in indwelling catheters and to compare three methods i.e. Congo red agar (CRA) method, Tissue culture plate (TCP) method, and tube method (TM). In this study, we evaluated the reliability of these methods in order to determine most suitable screening method and noted the sensitivity pattern of these isolates with the help of Vitek-2. Our data indicates that the TCP is an accurate and reproducible method for screening and can serve as a reliable quantitative tool for determining bio film formation by clinical isolates of Candida species along with susceptibility testing to reduce resistance pattern.

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Brevundimonas vesicularis is infrequently isolated from clinical sample in patient with or without immunosuppression. A 51 year old patient having diabetes with Coronary Artery Disease with Double Valve Disease and Peripheral Vascular Disease is described. The blood culture of the patient was positive after 24 hours by Biomerieux BacT/Alert 3D 60. Identified was done by API ID 32 G automated system. There is a little literature that supports isolation of B. vesicularis from a clinical specimen in India. After overview of literature, it is suggested that third generation cephalosporin, piperacillin/ tazobactum and ciprofloxacin is effective in treating infection caused by B. vesicularis, while efficacy of ampicillin sulbactum has to be studied further.